POSS nanocomposite hydrogel for 3D bioprinting

Patent metadata

Field	Value
Publication number	US-11541148-B2
Application number	US-201916398603-A
Country	US
Kind code	B2
Filing date	Apr 30, 2019
Priority date	Apr 30, 2018
Publication date	Jan 3, 2023
Grant date	Jan 3, 2023

Abstract

Provided is a bioink comprising a mixture comprising a collagen and a polysaccharide, and a polyhedral oligomeric silsesquioxane (POSS), a hydrogel matrix formed from a bioink comprising a mixture comprising a collagen and a polysaccharide, and a polyhedral oligomeric silsesquioxane (POSS), a 3D biomaterial scaffold comprising a hydrogel matrix of the disclosure as a first hydrogel layer and a hydrogel matrix of the disclosure as a second hydrogel layer, optionally having an intervening layer between the first hydrogel layer and the second hydrogel layer, and methods of forming and using same.

First claim

What is claimed:

1. A bioink, comprising:
an ink mixture comprising:
a collagen and a polysaccharide;
a polyhedral oligomeric silsesquioxane (POSS); and
a solvent for one or more of the collagen, polysaccharide, or POSS;

wherein the POSS is included in the bioink in an amount of about 0.05% to about 2.0% by weight, based on the total weight of non-solvent components of the bioink;
wherein when the ink mixture is printed at a temperature in a range of about 0° C. to about 37° C., the ink forms a gel within less than about 1 min.

2. The bioink of claim 1 , wherein the polysaccharide comprises alginate, hyaluronic acid, agarose, heparin, or a combination thereof.

3. The bioink of claim 1 , wherein the polysaccharide comprises alginate.

4. The bioink of claim 1 , wherein the polysaccharide is provided in an amount of 2.5 to 60 mg/mL solvent.

5. The bioink of claim 1 , wherein the collagen is provided in an amount of 1.5 to 12 mg/mL solvent.

6. The bioink of claim 1 , wherein the collagen and polysaccharide are provided in a ratio (collagen:polysaccharide) of 5:1 to 1:40 (w/w).

7. The bioink of claim 1 , wherein the POSS comprises a compound having a structure of Formula (I):

and
each R is independently H, C1-C20 alkyl, C3-C10 cycloalkyl, aryl, or C1-C20 alkoxyl, C1-C20 thiolalkyl, C1-C20 aminoalkyl.

8. The bioink of claim 7 , wherein at least one R comprises isobutyl.

9. The bioink of claim 1 , wherein the POSS comprises a compound having a structure of Formula (II):

and
each R is independently H, C1-C20 alkyl, C3-C10 cycloalkyl, aryl, or C1-C20 alkoxyl, C1-C20 thiolalkyl, C1-C20 aminoalkyl.

10. The bioink of claim 9 , wherein at least one R comprises phenyl.

11. The bioink of claim 9 , wherein R, collectively, has an average m of 13 to 14.

12. The bioink of claim 1 , wherein the POSS comprises a compound having a structure of Formula (III):

and
each R is independently —(CH 2 ) n (OCH 2 CH 2 ) m OCH 3 , each n is an integer of 1 to 5 and each m is an integer of 2 to 30.

13. The bioink of claim 1 , wherein the polysaccharide comprises alginate, the POSS comprises a compound having a structure of Formula (I):

each R is isobutyl, the bioink further comprises a solvent comprising water, the collagen is provided in an amount of 3 mg/mL solvent, the alginate is provided in an amount of 5 mg/mL solvent, and the POSS is provided in an amount of 0.1% to 1.0% (w/v), based on the total volume of the hydrogel.

14. The bioink of claim 1 , wherein the polysaccharide comprises alginate, the POSS comprises a compound having a structure of Formula (I):

each R is ethyl, the bioink further comprises a solvent comprising water, the collagen is provided in an amount of 3 mg/mL solvent, the alginate is provided in an amount of 5 mg/mL solvent, and the POSS is provided in an amount of 0.1% to 1.0% (w/v), based on the total volume of the hydrogel.

15. The bioink of claim 1 , wherein the polysaccharide comprises alginate, the POSS comprises a compound having a structure of Formula (I):

each R is phenyl, the bioink further comprises a solvent comprising ethanol, the collagen is provided in an amount of 3 mg/mL solvent, the alginate is provided in an amount of 5 mg/mL solvent, and the POSS is provided in an amount of 0.1% to 1.0% (w/v), based on the total volume of the hydrogel.

16. The bioink of claim 1 , wherein the polysaccharide comprises alginate, the POSS comprises a compound having a structure of Formula (II):

each R is phenyl, the bioink further comprises a solvent comprising ethanol, the collagen is provided in an amount of 3 mg/mL solvent, the alginate is provided in an amount of 5 mg/mL solvent, and the POSS is provided in an amount of 0.1% to 1.0% (w/v), based on the total volume of the hydrogel.

17. The bioink of claim 1 , wherein the polysaccharide comprises alginate, the POSS comprises a compound having a structure of Formula (III):

each R is independently —(CH2)n(OCH2CH2)mOCH3, n is 2, the average m is 13.3, the bioink comprises a solvent comprising ethanol, the collagen is provided in an amount of 3 mg/mL solvent, the alginate is provided in an amount of 5 mg/mL solvent, and the POSS is provided in an amount of 0.1% to 1.0% (w/v), based on the total volume of the hydrogel.

18. The bioink of claim 1 , further comprising a drug, growth factor, signaling group, fluorescent tag, or a combination thereof.

19. The bioink of claim 18 , wherein the drug, growth factor, signaling group, fluorescent dye, or combination thereof is covalently attached to the POSS.

20. The bioink of claim 1 , further comprising one or more of glycosaminoglycans, fibrin, laminin, and a combination thereof.

21. The bioink of claim 20 , wherein the one or more of glycosaminoglycans, fibrin, laminin, and a combination thereof is present in the bioink in an amount of 0.1 to 10 wt. %, based upon total weight of the bioink.

22. A hydrogel matrix formed from the bioink of claim 1 .

23. The hydrogel matrix of claim 22 , wherein the POSS is covalently bonded to the polysaccharide.

24. The hydrogel matrix of claim 22 , wherein the POSS is dispersed homogenously throughout the matrix.

25. They hydrogel matrix of claim 22 , wherein the hydrogel matrix is porous and has a pore size of 10 micron to 200 micron in diameter.

26. The hydrogel matrix of claim 25 , wherein at least 50% of pores are 90 to 150 micron in diameter.

27. A 3D biomaterial scaffold comprising
the hydrogel matrix of claim 22 as a first hydrogel layer and
the hydrogel matrix of claim 22 as a second hydrogel layer,
optionally having an intervening layer between the first hydrogel layer and the second hydrogel layer.

28. The 3D biomaterial scaffold of claim 27 , wherein the second hydrogel layer is directly adjacent to the first hydrogel layer.

29. The 3D biomaterial scaffold of claim 27 , further comprising one or more of live cells and a tissue embedded in the first hydrogel layer, the second hydrogel layer, or both.

30. The 3D biomaterial scaffold of claim 27 , further comprising one or more of live cells and a tissue seeded on the 3D biomaterial scaffold.

31. A method for sustained release of a drug to a patient in need thereof, comprising:
grafting the 3D biomaterial scaffold of claim 27 into a surgical site on the patient, wherein the 3D biomaterial scaffold comprises the drug, either dispersed within the 3D biomaterial scaffold or covalently attached to the POSS.

Assignees

• Univ Michigan Regents

Inventors

• Liu Chun
• Luker Kathryn
• Luker Gary

Classifications

• A61L27/24

  Collagen · CPC title

• A61L2300/414

  Growth factors · CPC title

• B33Y70/00

  Materials specially adapted for additive manufacturing · CPC title

• A61L27/56 Primary

  Porous materials, {e.g. foams or sponges} · CPC title

• B33Y80/00

  Products made by additive manufacturing · CPC title