Pesticidal genes and methods of use

Patent metadata

Field	Value
Publication number	US-11540520-B2
Application number	US-202017009339-A
Country	US
Kind code	B2
Filing date	Sep 1, 2020
Priority date	Jan 30, 2017
Publication date	Jan 3, 2023
Grant date	Jan 3, 2023

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and using those polypeptides for controlling or killing a pest.

First claim

That which is claimed is:

1. A recombinant nucleic acid molecule encoding a polypeptide comprising
an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity;
wherein said recombinant nucleic acid molecule is not a naturally occurring sequence encoding said polypeptide.

2. The recombinant nucleic acid molecule of claim 1 , wherein said recombinant nucleic acid molecule is operably linked to a promoter capable of directing expression in a plant cell.

3. A host cell comprising a nucleic acid molecule encoding a polypeptide comprising
an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

4. The host cell of claim 3 , wherein said host cell is a bacterial host cell or a plant cell.

5. A DNA construct comprising a heterologous promoter operably linked to a nucleic acid molecule that encodes a polypeptide comprising
an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

6. A vector comprising the DNA construct of claim 5 .

7. A host cell comprising the vector of claim 6 .

8. A transgenic plant comprising the host cell of claim 7 .

9. A formulation comprising a polypeptide, wherein the polypeptide comprises
an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

10. The formulation of claim 9 , wherein said formulation is selected from the group consisting of a powder, dust, pellet, granule, wettable granule, wettable powder, spray, emulsion, colloid, and solution.

11. A plant having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule that encodes a protein having pesticidal activity, wherein said nucleic acid molecule comprises
a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

12. A transgenic seed of the plant of claim 11 , wherein said seed has stably incorporated into its genome the DNA construct.

13. A method for controlling a pest population comprising contacting said pest population with a pesticidal-effective amount of the formulation of claim 9 .

14. A method for producing a polypeptide with pesticidal activity comprising culturing the host cell of claim 7 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

15. A method for protecting a plant from an insect pest, comprising expressing in a plant or cell thereof a nucleic acid molecule that encodes a pesticidal polypeptide, wherein said nucleic acid molecule comprises
a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

16. The method of claim 15 , wherein protecting said plant comprises controlling insect pest damage to said plant.

17. The method of claim 15 , wherein said plant produces a pesticidal polypeptide having pesticidal activity against at least one of a lepidopteran pest, a coleopteran pest, or a hemipteran pest.

18. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule that encodes a pesticidal polypeptide, wherein said nucleic acid molecule comprises
a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 89, wherein the polypeptide has pesticidal activity.

19. The DNA construct of claim 5 , wherein the promoter drives expression in a plant cell.

20. The DNA construct of claim 5 , wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant.

21. The DNA construct of claim 5 , wherein the promoter drives expression in a bacterial cell.

22. The plant of claim 11 , wherein said pesticidal activity controls a lepidopteran pest, a hemipteran pest, or a coleopteran pest.

23. The plant of claim 11 , wherein the plant is a monocot.

24. The plant of claim 11 , wherein the plant is a dicot.

25. The plant of claim 23 , wherein the plant is corn, sorghum, wheat, rice, sugarcane, barley, oats, rye, millet, coconut, pineapple or banana.

26. The plant of claim 24 , wherein the plant is sunflower, tomato, crucifers, peppers, potato, cotton, soybean, sugarbeet, tobacco, oilseed rape, sweet potato, alfalfa, safflower, peanuts, cassava, coffee, cocoa, cucumber, lettuce, olive, peas, or tea.

27. The recombinant nucleic acid molecule of claim 1 , wherein the recombinant nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

28. The host cell of claim 3 , wherein the nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

29. The DNA construct of claim 5 , wherein the nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

30. The formulation of claim 9 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 89.

31. The plant of claim 11 , wherein the nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

32. The method of claim 15 , wherein the nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

33. The method of claim 18 , wherein the nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 89.

Assignees

• Agbiome Inc

Inventors

• Parks Jessica
• Roberts Kira Bulazel
• Thayer Rebecca E

Classifications

• C07K14/195

  from bacteria · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8286

  for insect resistance · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/1086

  Preparation or screening of expression libraries, e.g. reporter assays · CPC title