Rapid identification and sequence analysis of intact proteins in complex mixtures

Patent metadata

Field	Value
Publication number	US-11538675-B2
Application number	US-201716307615-A
Country	US
Kind code	B2
Filing date	Jun 5, 2017
Priority date	Jun 6, 2016
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.

First claim

What is claimed is:

1. A method for m/z selectively arresting ion-ion reactions (ion parking) within the ion-ion reaction cell of a mass spectrometer system, said method comprising
providing a reaction cell comprising reagent ions and a set of electrodes with one or more periodic RF confinement voltages applied to them wherein during ion-ion reactions in one or more dimensions of ion motion, ions have oscillatory motion frequencies that are primarily determined by their mass-to-charge ratios,
applying one or more auxiliary voltage waveforms to one or more electrodes of the ion-ion reaction cell, during a period where ion-ion reactions are performed, such that a first set of frequency components of one or more of said auxiliary waveform voltages provides m/z selective kinetic excitation to the reagent ions,
kinetically exciting analyte product ions produced from said ion-ion reactions within one or more selected m/z ranges, during said period where ion-ion reactions are performed, so as to partially or fully arrest the further reaction of said analyte product ions within said selected m/z ranges;
wherein the one or more auxiliary voltage waveforms include a plurality of frequency components selected to kinetically excite, via resonant or near-resonant excitation, analyte product ions within the one or more selected m/z ranges, and a notch corresponding to the m/z of the analyte precursor ion.

2. The method of claim 1 wherein the ion-ion reaction cell is one of the following types of devices
a RF 2D quadrupole ion trap having one or more segments or sections
a RF 3D quuadrupole ion traps
a RF torriodal traps.

3. The method of claim 1 wherein the analyte product ions have a charge state of 9 or more charges.

Assignees

• Hunt Donald F
• English Ann M
• Ugrin Scott A
• Bai Dina L
• Shabanowitz Jeffrey
• Syka John Edward Philip
• Univ Virginia Patent Foundation
• Thermo Finnigan Llc

Inventors

• Hunt Donald F
• English Ann M
• Ugrin Scott A
• Bai Dina L
• Shabanowitz Jeffrey
• Syka John Edward Philip

Classifications

• H01J49/4295

  Storage methods · CPC title

• G01N33/6848

  Methods of protein analysis involving mass spectrometry · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/0072 Primary

  by ion/ion reaction, e.g. electron transfer dissociation, proton transfer dissociation · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title