System and method for flexibly representing and processing assay plates

Patent metadata

Field	Value
Publication number	US-11536737-B2
Application number	US-201816227041-A
Country	US
Kind code	B2
Filing date	Dec 20, 2018
Priority date	Mar 11, 2002
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

A flexible instrument control and data storage/management system and method for representing and processing assay plates having one or more predefined plate locations is disclosed. The system utilizes a graph data structure, layer objects and data objects. The layer objects map the graph data structure to the data objects. The graph data structure can comprise one node for each of the one or more predefined plate locations, wherein the nodes can be hierarchically defined according to a predefined plate location hierarchy. Each node can be given a unique node identifier, a node type and a node association that implements the predefined plate location hierarchy. The layer objects can include an index that maps the node identifiers to the data objects.

First claim

What is claimed is:

1. A method of controlling an instrument to process an assay plate, the assay plate comprising a plurality of wells, and each of the plurality of wells comprises a well point, the well point being a point inside each of the plurality of wells, the assay plate having associated geometry data that includes one or more predefined points of interest, the method employing a computerized set of instructions implementing a tree of one or more multi-dimensional coordinate space objects, wherein the tree has a root coordinate space and each coordinate space object has a plurality of subspaces, each of the subspaces being anchored in the coordinate space object at a predefined subspace anchor point and wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest, wherein each of the plurality of subspaces is associated with at least one of the plurality of wells, the method comprising:
ascertaining a point of interest of the one or more predefined points of interest;
resolving the point of interest to the root coordinate space as an ascertained point;
instructing the instrument to move to the ascertained point of interest by traversing the tree of coordinate spaces; and
instructing the instrument to move to a second point, wherein the second point is directly above the well point of one of the plurality of wells.

2. The method of claim 1 , further comprising aspirating contents of the at least one of the plurality of wells after the instrument moves to the second point.

3. The method of claim 1 , wherein each subspace is associated with one well of the plurality of wells.

4. The method of claim 1 , wherein the assay plate comprises 96 wells arranged in a grid of 8 rows and 12 columns of wells, wherein each well grouping is one column of wells.

5. The method of claim 1 , further comprising:
accessing a database comprising a plate type directory, which comprises a plurality of graph data structures and representing the assay plate in a graph data structure of the plurality of graph data structures, wherein the graph data structure comprises a node for each of the one or more predefined plate locations and said node is hierarchically arranged to correspond to the physical structure of the assay plate;
storing a least one data object having one or more data records, wherein the at least one data object is associated with the assay plate and the at least one data object is not stored on a database comprising the graph data structure; and
mapping the at least one data object to one or more nodes within the graph data structure via at least one layer object.

6. The method of claim 1 , wherein the plurality of wells are arranged in a plurality of well groupings, and wherein the one or more predefined plate locations include: one or more spots in each well; and one or more sectors associated with each grouping of wells, wherein the one or more sectors each contain the one or more spots, respectively, from each well of the associated group of wells.

7. The method of claim 5 , wherein the assay plate has associated geometry data that includes the well point and one or more points as one or more predefined points of interest, the database further comprises: a tree of one or more multi-dimensional coordinate space objects having a plurality of subspaces, the subspaces each being anchored in the coordinate space object at a predefined subspace anchor point, wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest.

8. A non-transitory computer readable medium having stored thereon a computer program which, when executed on a computing system operatively connected to an instrument to process an assay plate, the assay plate comprising a plurality of wells, and each of the plurality of wells comprises a well point, the well point being a point inside each of the plurality of wells, the assay plate having associated geometry data that includes one or more predefined points of interest, the method employing a computerized set of instructions implementing a tree of one or more multi-dimensional coordinate space objects, wherein the tree has a root coordinate space and each coordinate space object has a plurality of subspaces, each of the subspaces being anchored in the coordinate space object at a predefined subspace anchor point and wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest, wherein each of the plurality of subspaces is associated with at least one of the plurality of wells, the method comprising the steps of:
ascertaining a point of interest the one or more predefined points of interest;
resolving the point of interest to the root coordinate space as an ascertained point;
instructing the instrument to move to the ascertained point of interest by traversing the tree of coordinate spaces; and
instructing the instrument to move to a second point, wherein the second point is directly above the well point of one of the plurality of wells.

9. The non-transitory computer readable medium of claim 8 , wherein the method further comprises the step of:
aspirating contents of the at least one of the plurality of wells after the instrument moves to the second point.

10. The non-transitory computer readable medium of claim 8 , wherein each subspace is associated with one well of the plurality of wells.

11. The non-transitory computer readable medium of claim 8 , wherein the assay plate comprises 96 wells arranged in a grid of 8 rows and 12 columns of wells, wherein each well grouping is one column of wells.

12. The non-transitory computer readable medium of claim 8 , wherein the method further comprises the steps of:
accessing a database comprising a plate type directory, which comprises a plurality of graph data structures and representing the assay plate in a graph data structure of the plurality of graph data structures, wherein the graph data structure comprises a node for each of the one or more predefined plate locations and said node is hierarchically arranged to correspond to the physical structure of the assay plate;
storing a least one data object having one or more data records, wherein the at least one data object is associated with the assay plate and the at least one data object is not stored on a database comprising the graph data structure; and
mapping the at least one data object to one or more nodes within the graph data structure via at least one layer object.

13. The non-transitory computer readable medium of claim 8 , wherein the plurality of wells are arranged in a plurality of well groupings, and wherein the one or more predefined plate locations include: one or more spots in each well; and one or more sectors associated with each grouping of wells, wherein the one or more sectors each contain the one or more spots, respectively, from each well of the associated group of wells.

14. The non-transitory computer readable medium of claim 12 , wherein the assay plate has associated geometry data that includes the well point and one or more points as one or more predefined points of interest, the database further comprises: a tree of one or more multi-dimensional coordinate space objects having a plurality of subspaces, the subspaces each being anchored in the coordinate space object at a predefined subspace anchor point, wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest.

15. A system having at least one central processing unit (CPU), and at least one memory storage device having stored the

Assignees

• Meso Scale Technologies Llc

Inventors

• Lovell Craig P
• Hampton Iii Thomas Lucas

Classifications

• G01N35/00584

  Control arrangements for automatic analysers · CPC title

• Y10T436/11

  Automated chemical analysis · CPC title

• B01L9/50

  Clamping means, e.g. tongs · CPC title

• G01N35/028

  having reaction cells in the form of microtitration plates · CPC title

• B01L3/545

  for laboratory containers · CPC title