Method and device for the raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs
US-9835560-B2 · Dec 5, 2017 · US
US11536704B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11536704-B2 |
| Application number | US-201716344988-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 26, 2017 |
| Priority date | Oct 26, 2016 |
| Publication date | Dec 27, 2022 |
| Grant date | Dec 27, 2022 |
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To determine the sex of a bird egg, a hole is produced at the blunt end of the bird egg, wherein the hole affects the calcareous shell and the outer shell membrane, whereas the inner shell membrane remains intact. In the region of the hole at the blunt end, beneath the intact inner shell membrane, at least one blood vessel is registered and the blood therein is excited by means of a preset incident radiation, the back-scattered radiation of which blood is measured, detected and evaluated for the sex determination.
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The invention claimed is: 1. A method for positioning a measuring point on at least one blood-carrying vessel of an opened bird egg to subsequently determine the sex of the bird egg, with a production of a hole on the bird egg, which comprises at least a blunt end and a pointed end as well as an egg shell having an inner shell membrane and an outer shell membrane located more proximately to the calcareous shell of the bird egg, wherein the bird egg is opened in order to perform at least one optical measurement related to the blood, the method comprising: carrying out the production of the hole at the blunt end of the bird egg by means of a hole producer, wherein the hole affects the calcareous shell and the outer shell membrane, which forms an air space with an inner shell membrane, and the inner shell membrane remains intact, wherein in the region of the hole at the blunt end beneath the intact inner shell membrane at least one blood vessel is registered and the blood therein is excited by means of a preset incident radiation, with the back-scattered radiation of the blood by which radiation is conducted through the intact inner shell membrane and is related to the blood, being measured, detected and evaluated for determining the sex; twisting shafts that are rotatable/tiltable in an xy-plane by means of a motor-controlled tilt apparatus, which is connected to a central controller, so that should the position of the embryo deviate from the central egg axis the deviating position of the embryo is brought into the immediate region of the egg axis in a centered fashion and a minimum distance is obtained between the arch of the inner shell membrane and the embryo. 2. The method according to claim 1 , wherein: during the egg-opening, the calcareous shell provided with the adherent outer shell membrane, is perforated and the blood of at least one blood vessel is spectroscopically examined, and the inner shell membrane remains intact during the egg-opening and the subsequent spectroscopic optical measuring signal acquisition through at least the inner shell membrane of the egg shell. 3. The method according to claim 1 , wherein: before and during the measurement, a temperature control of the egg holder and the measurement environment of the egg are performed with an assigned temperature controller. 4. The method according to claim 1 , wherein: after the detection of the back-scattered radiation, the hole in the blunt end is closed by means of a seal, and at least the incubation of the egg for which the sex has been determined to be female is continued. 5. The method according to claim 1 , wherein: one or more lasers are used for registering a blood vessel, for optical excitations of the blood and/or for measurement of the back-scattered radiation related to the blood. 6. An apparatus for positioning a measuring point on at least one blood-carrying vessel of an opened bird egg within an egg tray for subsequently determining the sex of the bird egg, the apparatus comprising at least: a hole producer configured to produce a hole at the blunt end of the bird egg; an optical measurer configured to measure the back-scattered radiation related to the blood; two axes that can be rotated/tilted on an xy-plane, which axes are connected to the egg tray, wherein the axes are operated by rotation/tilt signals; a motor-controlled tilting apparatus that is connected to the axes; and a central controller that is connected to the motor-controlled tilting apparatus; wherein the central controller is configured to rotate the axes such that, if the embryo drifts out of the central egg axis, the drifting embryo is positioned in the proximate region of the egg axis in a centered manner and a minimum distance between the curvature of the inner shell membrane and the embryo is achieved. 7. The apparatus according to claim 6 , wherein: the connections between the motor-controlled tilting apparatus and the axes are at least embodied by connection lines, and the connections between the motor-controlled tilting apparatus and the central controller are at least embodied by connection lines.
Physical analysis of biological material · CPC title
Raman scattering · CPC title
Injecting or otherwise treating hatching eggs · CPC title
Eggs, e.g. by candling · CPC title
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