Electrochemical method and device for detecting the effect of anticancer drugs

Patent metadata

Field	Value
Publication number	US-11536684-B2
Application number	US-202016813781-A
Country	US
Kind code	B2
Filing date	Mar 10, 2020
Priority date	Mar 3, 2016
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

A biosensor for measuring an electrical response from a biological sample. The biosensor includes a substrate, a passivation layer grown on a surface of the substrate, a patterned catalyst layer deposited on the passivation layer, and three electrodes grown on the patterned catalyst layer. The three electrodes include a working electrode, a counter electrode, and a reference electrode. The working electrode includes a first array of electrically conductive biocompatible nanostructures that is configured to be an attachment site for the biological sample. The counter electrode includes a second array of electrically conductive biocompatible nanostructures that is configured to acquire the electrical response from the working electrode. The reference electrode includes a third array of electrically conductive biocompatible nanostructures that is configured to adjust a specific voltage around the working and the counter electrodes.

First claim

What is claimed is:

1. A biosensor for measuring an electrical response from a biological sample, the biosensor comprising:
a substrate;
a passivation layer, the passivation layer comprising an electrical passivating layer grown on a surface of the substrate;
a patterned catalyst layer comprising a patterned catalyst layer deposited on the passivation layer; and
three electrodes grown on the patterned catalyst layer, the three electrodes comprising:
a working electrode comprising a first array of electrically conductive biocompatible nanostructures, configured to be an attachment site for the biological sample;
a counter electrode comprising a second array of electrically conductive biocompatible nanostructures, configured to acquire the electrical response from the working electrode; and
a reference electrode comprising a third array of electrically conductive biocompatible nanostructures, configured to adjust a specific voltage around the working and the counter electrodes,
wherein the electrically conductive biocompatible nanostructures comprise at least one of silicon nanowires (SiNWs), silicon nanograss, carbon nanotubes (CNTs), and combinations thereof.

2. The biosensor according to claim 1 , wherein the substrate comprises a layer of silicon (Si).

3. The biosensor according to claim 1 , wherein the passivation layer comprises a layer of silicon dioxide (SiO 2 ) with a thickness in a range between 100 nm and 500 nm.

4. The biosensor according to claim 1 , wherein the patterned catalyst layer comprises a layer of at least one of nickel (Ni), gold (Au), iron (Fe), and combinations thereof.

5. The biosensor according to claim 1 , wherein the patterned catalyst layer comprises a layer of a catalyst with a thickness of less than 10 nm for growing electrically conductive biocompatible nanostructures thereon.

6. The biosensor according to claim 1 , wherein the patterned catalyst layer comprises one of a circular-patterned catalyst layer and an interdigital-patterned catalyst layer.

7. The biosensor according to claim 6 , wherein the circular-patterned catalyst layer comprises:
a first semi-circular layer of the catalyst, deposited and patterned on the passivation layer, the first semi-circular layer of the catalyst configured to grow the second array of electrically conductive biocompatible nanostructures thereon;
a second semi-circular layer of the catalyst, deposited and patterned on the passivation layer in front of the first semi-circular layer of the catalyst, the second semi-circular layer of the catalyst configured to grow the third array of electrically conductive biocompatible nanostructures thereon; and
a circular layer of the catalyst, deposited and patterned on the passivation layer between the first semi-circular layer of the catalyst and the second semi-circular layer of the catalyst, the circular layer of the catalyst configured to grow the first array of electrically conductive biocompatible nanostructures thereon.

8. The biosensor according to claim 6 , wherein the circular-patterned catalyst layer comprises:
a circular layer of the catalyst, deposited and patterned on the passivation layer, the circular layer of the catalyst configured to grow the first array of electrically conductive biocompatible nanostructures thereon;
a partially annular layer of the catalyst, deposited and patterned on the passivation layer around the circular layer of the catalyst, the partially annular layer of the catalyst configured to grow the second array of electrically conductive biocompatible nanostructures thereon; and
a reference-site layer of catalyst, deposited and patterned on the passivation layer adjacent to the circular layer of the catalyst and the partially annular layer of the catalyst, the reference-site layer of the catalyst configured to grow the third array of electrically conductive biocompatible nanostructures thereon.

9. The biosensor according to claim 6 , wherein the interdigital-patterned catalyst layer comprises:
a first teeth-shaped array of the catalyst layer, deposited and patterned on the passivation layer, the first teeth-shaped array of the catalyst layer configured to grow the first array of electrically conductive biocompatible nanostructures thereon;
a second teeth-shaped array of the catalyst layer, deposited and patterned on the passivation layer, the second teeth-shaped array of the catalyst layer configured to grow the second array of electrically conductive biocompatible nanostructures thereon; and
a singular layer of the catalyst, deposited and patterned on the passivation layer adjacent to the first teeth-shaped array of the catalyst layer and the second teeth-shaped array of the catalyst layer, the singular layer of the catalyst configured to grow the third array of electrically conductive biocompatible nanostructures thereon,

wherein the first teeth-shaped array of the catalyst layer and the second teeth-shaped array of the catalyst layer are placed inside each other.

10. The biosensor according to claim 9 , wherein each of the first teeth-shaped array of the catalyst layer and the second teeth-shaped array of the catalyst layer comprises an array of teeth-shaped catalyst layer with a distance between 5 μm and 200 μm for each respective two teeth patterned next to each other.

11. The biosensor according to claim 10 , wherein each tooth of the first teeth-shaped array of the catalyst layer and the second teeth-shaped array of the catalyst layer comprises a tooth with a width between 5 μm and 200 μm.

12. The biosensor according to claim 11 , wherein:
each tooth of the first teeth-shaped array of the catalyst layer is placed next to at least one tooth of the second teeth-shaped array of the catalyst layer, and
a distance between each tooth of the first teeth-shaped array of the catalyst layer and an adjacent tooth of the second teeth-shaped array of the catalyst layer is the same as the width of each teeth.

13. The biosensor according to claim 1 , wherein the SiNWs comprise a plurality of SiNWs with a width of less than 80 nm and a length of less than 50 μm.

14. The biosensor according to claim 1 , wherein the CNTs comprise a plurality of vertically aligned multi-walled carbon nanotubes (VAMWCNTs) with a length of between 0.5 μm and 10 μm and a diameter of between 20 nm and 100 nm.

Assignees

• Abdolahad Mohammad
• Shashaani Hani
• Faramarzpour Mahsa
• Nano Hesgarsazan Salamat Arya

Inventors

• Abdolahad Mohammad
• Shashaani Hani
• Faramarzpour Mahsa

Classifications

• G01N27/4075

  Composition or fabrication of the electrodes and coatings thereon, e.g. catalysts · CPC title

• G01N33/5438

  Electrodes · CPC title

• G01N27/3278 Primary

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• G01N27/4146

  involving nanosized elements, e.g. nanotubes, nanowires · CPC title

• G01N27/3272 Primary

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title