Partial enzymatic hydrolysis of triacylglycerols to produce long-chain polyunsaturated fatty acid

Patent metadata

Field	Value
Publication number	US-11535875-B2
Application number	US-201616066587-A
Country	US
Kind code	B2
Filing date	Dec 29, 2016
Priority date	Dec 30, 2015
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

Disclosed herein are host cells expressing a polypeptide, wherein the polypeptide hydrolyzes an ester linkage of a triacylglycerol in an oil comprising at least one long-chain polyunsaturated fatty acid, methods for using such host cells, and processes for production of a lipase using such host cells.

First claim

What is claimed is:

1. A method of obtaining at least one long-chain polyunsaturated fatty acid (LC-PUFA) from a triacylglycerol in an oil that comprises the at least one LC-PUFA, wherein said LC-PUFA comprises eicosapentaenoic acid (EPA), docosahexaneoic acid (DHA), or combinations thereof; the method comprising: hydrolyzing at least one ester linkage in said triacylglycerol with a lipase, wherein said lipase has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3.

2. The method of claim 1 , wherein the lipase is obtained by expressing it in a Pichia pastoris host cell.

3. The method of claim 1 or claim 2 , wherein the lipase has a 90% to 99% sequence identity to the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3.

4. The method of claim 1 , wherein the lipase has the amino acid sequence of SEQ ID NO: 1.

5. The method of claim 1 , wherein the lipase has the amino acid sequence of SEQ ID NO:2.

6. The method of claim 1 , wherein the lipase has the amino acid sequence of SEQ ID NO:3.

7. The method of claim 1 , wherein the oil is a crude or unrefined oil.

8. The method of claim 1 , wherein the oil is a marine oil.

9. The method according to claim 2 , wherein said Pichia pastoris host cell is transformed with a nucleic acid construct comprising a polynucleotide encoding the lipase.

Assignees

• Dsm Ip Assets Bv

Inventors

• Mugford Paul
• Mueller Monika
• Schurmann Martin

Classifications

• C12N9/20 Primary

  Triglyceride splitting, e.g. by means of lipase · CPC title

• C11C1/045

  using enzymes or microorganisms, living or dead · CPC title

• C12P7/6472 Primary

  containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone · CPC title

• C12N15/815

  for yeasts other than Saccharomyces · CPC title