Microorganism strain for high-performance metabolism of biomass-derived carbon source

Patent metadata

Field	Value
Publication number	US-11535854-B2
Application number	US-201816612300-A
Country	US
Kind code	B2
Filing date	May 11, 2018
Priority date	May 11, 2017
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

The present invention relates to a novel microorganism capable of metabolizing various carbon sources at high rates. A novel microorganism according to the present invention was observed to grow at a very high rate in a minimal medium/nutrient medium, etc., compared to microorganisms such as Escherichia coli, and shows resistance at a high initial sugar/salt concentrations as well as being able to produce lycopene and 2,3-butanediol through genetic manipulation. Therefore, the novel microorganism can be used in various production fields of high value-added compounds using microorganisms.

First claim

What is claimed is:

1. A transformed Vibrio sp. DHG strain with improved metabolic efficiency for carbon sources,
wherein the transformed Vibrio sp. DHG strain is obtained by introducing a SXT recombination system expression cassette into a Vibrio sp. DHG strain having accession number KCTC13239BP;
wherein the Vibrio sp. DHG strain having accession number KCTC13239BP comprises a 16S rDNA gene consisting of the nucleotide sequence of SEQ ID NO: 1;
wherein the SXT recombination system expression cassette comprises a synthetic 5′ UTR (untranslated region) consisting of the nucleotide sequence of SEQ ID NO: 57; a promoter consisting of the nucleotide sequence selected from the group consisting of SEQ ID NOs: 22 to 35 and 56; and a gamma gene consisting of the nucleotide sequence of SEQ ID NO: 6; and
wherein the carbon source comprises at least one selected from the group consisting of glucose, mannitol, sucrose, arabinose, galactose, glycerol and alginic acid.

2. The transformed Vibrio sp. DHG strain of claim 1 ,
wherein the Vibrio sp. DHG strain having accession number KCTC13239BP comprises a beta gene consisting of the nucleotide sequence of SEQ ID NO: 3 or a beta protein consisting of the amino acid sequence of SEQ ID NO: 2.

3. The transformed Vibrio sp. DHG strain of claim 1 ,
wherein the Vibrio sp. DHG strain having accession number KCTC13239BP comprises an exo gene consisting of the nucleotide sequence of SEQ ID NO: 5 or an exo protein consisting of the amino acid sequence of SEQ ID NO: 4.

4. A transformed strain for lycopene production,
wherein a crtEBI gene consisting of the nucleotide sequence of SEQ ID NO: 9 is further introduced to the transformed Vibrio sp. DHG strain of claim 1 .

5. The transformed strain for lycopene production of claim 4 , wherein an idi gene consisting of the nucleotide sequence of SEQ ID NO: 10 is further introduced thereinto.

6. The transformed strain for lycopene production of claim 4 , wherein an ispA gene consisting of the nucleotide sequence of SEQ ID NO: 11 is further introduced thereinto.

7. The transformed strain for lycopene production of claim 4 , wherein a dxs gene consisting of the nucleotide sequence of SEQ ID NO: 12 is further introduced thereinto.

8. A transformed strain for producing 2,3-butanediol, wherein the transformed strain is obtained by introducing, into the transformed Vibrio sp. DHG strain of claim 1 , at least one gene selected from the group consisting of:
a budA gene consisting of the nucleotide sequence of SEQ ID NO: 13;
a budB gene consisting of the nucleotide sequence of SEQ ID NO: 14; and
a budC gene consisting of the nucleotide sequence of SEQ ID NO: 15.

9. The transformed strain for producing 2,3-butanediol of claim 8 , wherein the transformed strain is obtained by deleting, from the transformed Vibrio sp. DHG strain, at least one gene selected from the group consisting of:
a IdhA gene consisting of the nucleotide sequence of SEQ ID NO: 16;
a frdA gene consisting of the nucleotide sequence of SEQ ID NO: 17;
a frdB gene consisting of the nucleotide sequence of SEQ ID NO: 18;
a frdC gene consisting of the nucleotide sequence of SEQ ID NO: 19;
a frdD gene consisting of the nucleotide sequence of SEQ ID NO: 20;
a pflB gene consisting of the nucleotide sequence of SEQ ID NO: 21.

10. A method of producing lycopene, the method comprising culturing the transformed strain for lycopene production of claim 4 .

11. A method for producing 2,3-butanediol, the method comprising culturing the transformed strain for producing 2,3-butanediol of claim 8 .

Assignees

• Postech Acad Ind Found
• Seoul Nat Univ R&Db Foundation
• Seoul Nat Univ R&Db Foundation

Inventors

• Jung Gyoo Yeol
• Kwak Dong Hun
• Seo Sang Woo
• Lim Hyun Gyu

Classifications

• C12Y306/03001

  Phospholipid-translocating ATPase (3.6.3.1), i.e. Mg2+-ATPase · CPC title

• C12P23/00

  Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes (containing heterorings C12P17/00) · CPC title

• C12N15/74 Primary

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12R2001/625

  Streptoverticillium · CPC title