Medium material for removing phenol contamination from groundwater, method of producing the same, and use of the same

Patent metadata

Field	Value
Publication number	US-11535543-B2
Application number	US-202016823330-A
Country	US
Kind code	B2
Filing date	Mar 19, 2020
Priority date	Mar 28, 2019
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

A medium material for removing phenol contamination from groundwater, a method of producing the same, and use of the same id disclosed. In at least some embodiments, the medium material is a granular material which has an average particle diameter of 0.5-1.5 cm and is formed from a bacteria-entrapping solution, a manganese sand filter material, modified bentonite, and biochar at a mass ratio of 1:0.2-0.4:0.2-0.4:0.1-0.2 by a series of processes including strain culturing, catalysis, mixing, solidification, and the like. The medium material can remove phenol from groundwater, is a safe and environment-friendly material, has a long service life, and/or achieves waste treatment with waste.

First claim

What is claimed is:

1. A method of producing a medium material for removing phenol contamination from groundwater, comprising:
(a) weighing an amount of organic bentonite and an amount of cupric nitrate at a mass ratio of 1:0.25, respectively, formulating a cupric nitrate solution, adding said amount of organic bentonite to said cupric nitrate solution, withdrawing said amount of organic bentonite and placing it into a heating equipment to increase the temperature to 250-300° C. at 10° C/min, and roasting for 30-45 min to obtain a modified bentonite;
(b) culturing phenol-degrading bacteria extracted from a manganese sand filter material in a tryptic soy broth (TSB) medium with a concentration of 1±0.4% under ambient conditions of pH=6.0-7.3, 25° C., and a dissolved oxygen concentration of 8.15-8.40 mg/L for 10-24h to form an active bacterial agent;
(c) adjusting an OD600 value of said active bacterial agent to near 1.0, inoculating to 100 mL of an MSVP medium containing 0.20-0.45 g/L of SiO2 powder, and adding to 100 mL of a sodium alginate solution with a mass concentration of 0.5 g/L at a volume ratio of 10-20% after 15-24 h of adsorption to obtain a bacteria-entrapping solution; and
(d) adding said bacteria-entrapping solution, said manganese sand filter material, said modified bentonite, and an amount of biochar at a mass ratio of 1:(0.2-0.4):(0.2-0.4):(0.1-0.2) to a CaC12 crosslinking solution to form agglomerates or spheres through crosslinking and coagulation, which are washed with sterile water to obtain immobilized granules or prills.

2. The method of claim 1 , wherein said manganese sand filter material is a waste manganese sand filter material produced in a manganese-removing filtration tank of a waterworks.

3. The method of claim 1 , wherein said manganese sand filter material is preliminarily freeze-dried and sieved to have an average particle diameter of 50±10 μm in step (d).

4. The method of claim 1 , wherein said biochar is straw biochar or bagasse biochar having an average particle diameter of 80±10 μm.

5. The method of claim 1 , wherein said immobilized granules or prills have an average particle diameter of 0.5-1.5 cm.

6. A granular medium material for removing phenol contamination from groundwater, formed by mixing a bacteria-entrapping solution, a manganese sand filter material, a modified bentonite, and a biochar at a mass ratio of 1:(0.2-0.4):(0.2-0.4):(0.1-0.2),
wherein said medium material has an average particle diameter of 0.5-1.5 cm,
wherein the modified bentonite is obtained by weighing an amount of organic bentonite and an amount of cupric nitrate at a mass ratio of 1:0.25, respectively, formulating a cupric nitrate solution, adding said amount of organic bentonite to said cupric nitrate solution, withdrawing said amount of organic bentonite and placing it into a heating equipment to increase the temperature to 250-300° C. at 10° C/min, and roasting for 30-45 min, and wherein the bacteria-entrapping solution is obtained by culturing phenol-degrading bacteria extracted from a manganese sand filter material in a tryptic soy broth (TSB) medium with a concentration of 1±0.4% under ambient conditions of pH=6.0-7.3, 25° C., and a dissolved oxygen concentration of 8.15-8.40 mg/L for 10-24h to form an active bacterial agent, adjusting an OD600 value of said active bacterial agent to near 1.0, inoculating to 100 mL of an MSVP medium containing 0.20-0.45 g/L of SiO2 powder, and adding to 100 mL of a sodium alginate solution with a mass concentration of 0.5 g/L at a volume ratio of 10-20% after 15-24 h of adsorption.

7. The medium material for removing phenol contamination from groundwater according to claim 6 , wherein said manganese sand filter material is a waste manganese sand filter material produced in a manganese-removing filtration tank of a waterworks;
said biochar has an average particle diameter of 0.5-1 mm.

8. The medium material for removing phenol contamination from groundwater according to claim 6 , wherein said medium material has a removal rate between 90% and 95% for phenol.

9. A medium material for removing phenol contamination from groundwater produced by the method according to claim 1 .

10. The medium material of claim 9 , wherein said medium material has a removal rate between 90% and 95% for phenol.

11. The method of claim h further comprising using said medium material for removing phenol contamination from groundwater.

Assignees

• Chinese Res Acad Env Sciences

Inventors

• Jiang Yonghai
• Lian Xinying
• Xi Beidou
• Yang Yu
• Jia Yongfeng
• Xia Fu
• Han Xu

Classifications

• C02F2101/345

  Phenols · CPC title

• C02F3/34 Primary

  characterised by the microorganisms used · CPC title

• C02F3/348 Primary

  characterised by the way or the form in which the microorganisms are added or dosed · CPC title

• C02F2103/06

  Contaminated groundwater or leachate · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title