Enzyme immobilization in hierarchical metal-organic frameworks

Patent metadata

Field	Value
Publication number	US-11530404-B2
Application number	US-201716306563-A
Country	US
Kind code	B2
Filing date	May 25, 2017
Priority date	Jun 3, 2016
Publication date	Dec 20, 2022
Grant date	Dec 20, 2022

Abstract

Enzyme-immobilizing MOFs and methods for their use in enzymatically catalyzed reactions are provided. The MOFs are channel-type MOFs that present a hierarchical pore structure comprising a first set of large channels sized for enzyme immobilization and a second set of smaller channels running alongside of the large channels that remain enzyme-free and allow for reactant delivery to the enzymes and product expulsion from the larger channels.

First claim

What is claimed is:

1. An enzyme-immobilizing metal-organic framework compound of comprising:
a channel-type metal-organic framework compound comprising Zr 6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a csq-net topology in which the large channels have a hexagonal cross-section and the small channels have a triangular cross-section; and
catalytically active enzymes immobilized in the large channels.

2. The enzyme-immobilizing metal-organic framework compound of claim 1 , wherein the smaller channels are free of the catalytically active enzymes.

3. The enzyme-immobilizing metal-organic framework compound of claim 1 , wherein the Zr 6 cluster nodes are connected by tetratopic organic linkers.

4. The enzyme-immobilizing metal-organic framework compound of claim 3 , wherein the tetratopic organic linkers comprise pyrene groups.

5. The enzyme-immobilizing metal-organic framework compound of claim 3 , wherein the tetratopic organic linkers comprise parylene groups.

6. The enzyme-immobilizing metal-organic framework compound of claim 3 , wherein the tetratopic organic linkers comprise porphyrin groups.

7. The enzyme-immobilizing metal-organic framework compound of claim 1 having an enzyme loading of at least 10 weight percent.

8. An enzyme-immobilizing metal-organic framework compound of comprising:
a channel-type metal-organic framework compound comprising Zr 6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a length in the range from 100 nm to 1000 nm; and
catalytically active enzymes immobilized in the large channels.

9. The enzyme-immobilizing metal-organic framework compound of claim 8 , wherein the large channels have diameters in the range from 2 nm to 8 nm, the small channels have side lengths in the range from 0.5 nm to 5 nm, and the openings have heights and widths in the range from 0.5 nm to 3 nm.

10. A method of enzymatically catalyzing a reaction using enzyme-immobilizing metal-organic framework compounds comprising:
channel-type metal-organic framework compound comprising Zr 6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a csq-net topology in which the large channels have a hexagonal cross-section and the small channels have a triangular cross-section; and
catalytically active enzymes immobilized in the large channels of the channel-type metal-organic framework compounds,
the method comprising: exposing the enzyme-immobilizing metal-organic framework compounds to a sample comprising chemical reactants and allowing the immobilized enzymes to catalyze a reaction between the reactants to form one or more reaction products.

11. The method of claim 10 , wherein the smaller channels are free of the catalytically active enzymes.

12. The method of claim 10 , wherein the Zr 6 cluster nodes are connected by tetratopic organic linkers.

13. The method of claim 10 , wherein the tetratopic organic linkers comprise pyrene groups.

14. The method of claim 10 , wherein the tetratopic organic linkers comprise parylene groups.

15. The method of claim 10 , wherein the tetratopic organic linkers comprise porphyrin groups.

16. The method of claim 10 , wherein the large channels have diameters in the range from 2 nm to 8 nm, the small channels have side lengths in the range from 0.5 nm to 5 nm, and the openings have heights and widths in the range from 0.5 nm to 3 nm.

17. The method of claim 10 , wherein the enzyme-immobilizing metal-organic framework compounds have an enzyme loading of at least 10 weight percent.

18. The method of claim 10 , wherein the channel-type metal-organic framework compounds have lengths in the range from 100 nm to 1000 nm.

Assignees

• Univ Northwestern

Inventors

• Farha Omar K
• Li Peng
• Modica Justin A
• Mrksich Milan
• Hupp Joseph T

Classifications

• C12N11/02 Primary

  Enzymes or microbial cells immobilised on or in an organic carrier · CPC title

• C12P9/00

  Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen {(phosphoglycerides, C12P7/6481)} · CPC title

• A62D3/02

  by biological methods, i.e. processes using enzymes or microorganisms · CPC title

• C12N9/18

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• C12Y301/01074

  Cutinase (3.1.1.74) · CPC title