System and method for transferring tissue

Patent metadata

Field	Value
Publication number	US-11530380-B2
Application number	US-202117140459-A
Country	US
Kind code	B2
Filing date	Jan 4, 2021
Priority date	Jul 12, 2017
Publication date	Dec 20, 2022
Grant date	Dec 20, 2022

Abstract

A transfer device designed to extract an amorphous or semi-solid structure, tissue, or construct from supporting media while maintaining the spatial integrity/organizational architecture thereof. The transfer device can include a controller, an actuator assembly, a plunger, and a needle. The controller can move the transfer device and the plunger independently.

First claim

What is claimed is:

1. A transfer system for transferring a tissue structure resting in growth media from a first location of at least two locations to a second location of the at least two locations, the transfer system comprising:
a needle;
a transfer device operably coupled with the needle, the transfer device configured to move the needle towards and away from the tissue structure, the transfer device configured to move the needle to a pre-selected depth into the growth media, the transfer device configured to move from the first location to the second location; and
a controller operably coupled with the transfer device, the controller directing the transfer device move the needle, the needle withdrawing the tissue structure at the first location, the needle depositing the tissue structure at the second location, the controller electronically directing the transfer device to move from the first location after the needle withdraws the tissue structure and to the second location where the needle deposits the tissue structure.

2. The transfer system as in claim 1 wherein the transfer device comprises: a back plate including at least one fastening cavity;
a hub retention yoke operably coupled with the at least one fastening cavity;
a linear actuator operably coupled with the at least one fastening cavity, the linear actuator being directed by the controller;
a plunger mount operably coupled with the linear actuator enabling movement of a plunger, the plunger operably coupled with the needle, the plunger mount operably coupled with a mounting cavity; and
a syringe pump operably coupled with the plunger, the syringe pump moving the plunger.

3. The transfer system as in claim 1 wherein the needle operable coupling with a barrel.

4. The transfer system as in claim 1 wherein the needle comprises: a needle bore sized according to characteristics of the tissue structure being transferred.

5. The transfer system as in claim 3 further comprising: a coupler hub maintaining the barrel and the needle in a desired position.

6. The transfer system as in claim 5 further comprising: at least one o-ring surrounding the coupler hub, the at least one o-ring maintaining a fluid-resistant seal.

7. The transfer system as in claim 5 wherein the hub retention yoke comprises: at least one ear surrounding the coupler hub.

8. The transfer system as in claim 2 wherein the mounting cavity comprises threading.

9. The transfer system as in claim 2 further comprising a motor driving the linear actuator.

10. The transfer system as in claim 9 further comprising a helical flexible shaft coupling, a linear drive adapter, and a motor mount coupling the motor with the linear actuator.

11. The transfer system as in claim 9 further comprising a junction housing mounting the motor.

12. The transfer system as in claim 2 wherein the back plate comprises: edging ribs including alignment features for mounting the linear actuator and the hub retention yoke.

13. The transfer system as in claim 12 wherein the edging ribs comprise raised features, the raised features against which the hub retention yoke can rest.

14. The transfer system as in claim 12 wherein the edging ribs comprise: actuator stops upon which the linear actuator rests.

15. The transfer system as in claim 1 wherein the first location comprises a bioreactor.

16. The transfer system as in claim 1 wherein the second location comprises a bioreactor.

Assignees

• Deka Products Lp

Inventors

• Tilley Michael C
• Andrews Richard E
• Fawkes Dane C

Classifications

• C12N5/0062

  General methods for three-dimensional culture · CPC title

• C12M33/00

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title

• C12N2513/00

  3D culture · CPC title

• A61L27/3608

  Bone, e.g. demineralised bone matrix [DBM], bone powder · CPC title

• C12M21/08 Primary

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title