Insecticidal proteins from plants and methods for their use

Patent metadata

Field	Value
Publication number	US-11525144-B2
Application number	US-201916980087-A
Country	US
Kind code	B2
Filing date	Mar 12, 2019
Priority date	Mar 14, 2018
Publication date	Dec 13, 2022
Grant date	Dec 13, 2022

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed is:

1. A recombinant polypeptide comprising an insecticidal amino acid sequence having at least 90% sequence identity to SEQ ID NO: 16, and
a heterologous signal sequence, a histidine tag, or heterologous transit sequence operably linked to said insecticidal amino acid sequence.

2. The recombinant polypeptide of claim 1 , wherein said recombinant polypeptide is insecticidal against a Lepidopteran agricultural pest.

3. The recombinant polypeptide of claim 1 , wherein said heterologous signal sequence, histidine tag, or heterologous transit sequence is operably linked to the N-terminus of the insecticidal amino acid sequence.

4. The recombinant polypeptide of claim 1 , wherein said insecticidal amino acid sequence has at least 98% sequence identity to SEQ ID NO: 16.

5. An agricultural composition comprising at least one recombinant polypeptide of claim 1 .

6. A recombinant polynucleotide comprising
a nucleic acid encoding a recombinant polypeptide that comprises an insecticidal amino acid sequence having at least 90% sequence identity to SEQ ID NO: 16; and
a heterologous regulatory element, wherein said heterologous regulatory element is operably linked to the nucleic acid encoding said recombinant polypeptide.

7. The recombinant polynucleotide of claim 6 , wherein the heterologous regulatory element is a promoter.

8. The recombinant polynucleotide of claim 6 , wherein the nucleic acid encoding said recombinant polypeptide comprises codons optimized for expression in plants.

9. The recombinant polynucleotide of claim 6 , wherein the nucleic acid encoding said recombinant polypeptide is a cDNA.

10. A recombinant polynucleotide encoding the recombinant polypeptide of claim 1 .

11. A DNA construct comprising the recombinant polynucleotide of claim 10 .

12. A transgenic plant comprising the recombinant polynucleotide of claim 6 .

13. A transgenic plant comprising the DNA construct of claim 11 .

14. A method of inhibiting growth or killing an insect pest or pest population comprising contacting the insect pest with the recombinant polypeptide of claim 1 .

15. A method of controlling insect pest damage to plants comprising providing the transgenic plant of claim 12 to said insect pest or pest population for ingestion.

16. A method of inhibiting growth or killing an insect pest or pest population comprising expressing in a transgenic plant the recombinant polynucleotide of claim 6 and exposing said insect pest or pest population to said transgenic plant.

17. A method for controlling an insect pest infestation comprising providing in the diet of the pest the transgenic plant of claim 13 or a part thereof.

18. A method for improving the yield of a crop comprising growing the transgenic plant of claim 13 , wherein the yield of the crop is increased in the presence of an insect pest relative to the crop not comprising said transgenic plant.

19. The method of claim 15 , wherein the transgenic plant is selected from corn, soybean, wheat, rice, sorghum, sunflower, canola, barley, sugarcane, potatoes, tomatoes, cotton, rape seed, peanut, and alfalfa.

20. The method of claim 14 , wherein the insect pest or insect pest population is in the Order Lepidopteran.

21. The method of claim 20 , wherein the insect pest or insect pest population is corn earworm, European corn borer, fall armyworm, soybean looper, and velvet bean caterpillar.

Assignees

• Pioneer Hi Bred Int
• Hexima Ltd

Inventors

• Liu Lu
• Lum Amy
• Ong Azalea S
• Schepers Eric
• Udranszky Ingrid
• Zhong Xiaohong

Classifications

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K14/415

  from plants · CPC title

• A01N37/46 Primary

  N-acyl derivatives · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title