Devices and methods for contactless dielectrophoresis for cell or particle manipulation

Patent metadata

Field	Value
Publication number	US-11519877-B2
Application number	US-201816058478-A
Country	US
Kind code	B2
Filing date	Aug 8, 2018
Priority date	Mar 9, 2009
Publication date	Dec 6, 2022
Grant date	Dec 6, 2022

Abstract

Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

First claim

What is claimed is:

1. A dielectrophoresis device
comprising: a sample channel for receiving a sample;
a first electrode channel filled with a first electrode comprising a first conductive solution;
a first electrical insulation barrier between the first electrode channel and the sample channel, wherein the first electrical insulation barrier is adapted to electrically insulate the first electrode channel from the sample channel;
a second electrode channel filled with a second electrode comprising a second conductive solution;
a second electrical insulation barrier between the second electrode channel and the sample channel, wherein the second electrical insulation barrier is adapted to electrically insulate the second electrode channel from the sample channel; and
at least one insulating structure within the sample channel,

wherein the sample channel, the first electrode channel, and the second electrode channel are formed in a same layer of the device, the layer has a layer first surface and a layer second surface opposite the layer first surface, and the sample channel, the first electrode channel, and the second electrode channel are formed as a recess into the layer first surface.

2. The dielectrophoresis device of claim 1 , wherein the at least one insulating structure is separate from each of the first and second insulation barriers.

3. The dielectrophoresis device of claim 1 , wherein the same layer is a single substrate layer and the sample channel, the first electrode channel, the second electrode channel, and the at least one insulating structure are all formed in the single substrate layer, and wherein the first insulation barrier, the second insulation barrier, and the at least one insulating structure are formed by the single substrate layer.

4. The dielectrophoresis device of claim 3 , wherein the single substrate layer is made from at least one of polydimethylsiloxane, glass, polyimide, polycarbonate, silicon, and plastic.

5. The dielectrophoresis device of claim 1 , wherein the at least one insulating structure includes at least one of a circular shape, a diamond shape, a square shape, an angled shape, a rounded rectangle shape, a ridge shape, an oval shape, and a bump shape.

6. The dielectrophoresis device of claim 5 , further comprising a plurality of insulating structures.

7. The dielectrophoresis device of claim 6 , wherein the plurality of insulating structures are arranged in a plurality of rows and/or columns.

8. The dielectrophoresis device of claim 7 , wherein the plurality of insulating structures are equally spaced from each other.

9. The dielectrophoresis device of claim 7 , wherein the plurality of insulating structures are unequally spaced from each other.

10. The dielectrophoresis device of claim 7 , wherein at least one of the plurality of insulating structures is circular shaped and has a diameter of about 100 micrometers.

11. The dielectrophoresis device of claim 1 , wherein the first and second electrical insulation barriers are made from at least one of polydimethylsiloxane, glass, polyimide, polycarbonate, silicon, and plastic.

12. The dielectrophoresis device of claim 1 , wherein a portion of the sample channel is linear.

13. The dielectrophoresis device of claim 12 , wherein a width of the sample channel changes over a length of the sample channel.

14. The dielectrophoresis device of claim 13 , wherein the width of the sample channel is narrower at a first end and/or second end of the sample channel than a middle of the sample channel along the length of the sample channel.

15. The dielectrophoresis device of claim 1 , wherein the first and second conductive solutions are selected from the group consisting of: phosphate buffer saline, a conductive gel, a conductive paint, a polyelectrolyte, a conductive ink, a conductive epoxy, and a conductive glue.

16. The dielectrophoresis device of claim 1 , wherein the at least one of the plurality of insulating structures extends from a bottom of the sample channel to the layer first surface.

17. A dielectrophoresis device comprising:
a sample channel for receiving a sample;
a first electrode channel for receiving a first electrode;
a first electrical insulation barrier between the first electrode channel and the sample channel, wherein the first electrical insulation barrier is adapted to electrically insulate the first electrode channel from the sample channel;
a second electrode channel for receiving a second electrode;
a second electrical insulation barrier between the second electrode channel and the sample channel, wherein the second electrical insulation barrier is adapted to electrically insulate the second electrode channel from the sample channel; and
a plurality of insulating structures, wherein the plurality of insulating structures is within the sample channel,

wherein the sample channel, the first electrode channel, and the second electrode channel are formed in a same layer of the device, the layer has a layer first surface and a layer second surface opposite the layer first surface, and the sample channel, the first electrode channel, and the second electrode channel are formed as a recess into the layer first surface,
wherein the at least one of the plurality of insulating structures includes at least one of a circular shape, a diamond shape, a square shape, an angled shape, a rounded rectangle shape, a ridge shape, an oval shape, and a bump shape, and
wherein at least two of the plurality of insulating structures are a different size from each other.

18. The dielectrophoresis device of claim 17 , wherein the first and second electrode channels are filled with a conductive solution.

19. The dielectrophoresis device of claim 17 , wherein the at least one of the plurality of insulating structures is separate from each of the first and second insulation barriers.

20. The dielectrophoresis device of claim 17 , wherein the same layer is a single substrate layer and the sample channel, the first electrode channel, the second electrode channel, and the at least one of the plurality of insulating structures are all formed in the single substrate layer, and wherein the first insulation barrier, the second insulation barrier, and the at least one of the plurality of insulating structures are formed by the single substrate.

21. The dielectrophoresis device of claim 20 , wherein the single substrate layer is made from at least one of polydimethylsiloxane, glass, polyimide, polycarbonate, silicon, and plastic.

22. The dielectrophoresis device of claim 17 , wherein the first and second electrical insulation barriers are made from at least one of polydimethylsiloxane, glass, polyimide, polycarbonate, silicon, and plastic.

23. The dielectrophoresis device of claim 17 , wherein a portion of the sample channel is linear.

24. The dielectrophoresis device of claim 23 , wherein a width of the sample channel changes over a length of the sample channel.

25. The dielectrophoresis device of claim 24 , wherein the width of the sample channel is narrower at a first end and/or second end of the sample channel than a middle of the sample channel along the length of the sample channel.

26. The dielectrophoresis device of claim 17 , wherein the first and second electrode channels are filled with the first and second electrodes, wherein the first and second electrodes comprise a conductive solution selected from the

Assignees

• Virginia Tech Intellectual Properties Inc
• Virginia Polytechnic Institute And State Univ

Inventors

• Davalos Rafael V
• Shafiee Hadi
• Sano Michael Benjamin
• Caldwell John L

Classifications

• B03C5/022

  Non-uniform field separators · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B01D57/02

  by electrophoresis (treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26) · CPC title

• B03C5/026

  using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC] · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title