Method for producing double-stranded RNA-rich lactic acid bacterium, and said lactic acid bacterium

Patent metadata

Field	Value
Publication number	US-11518976-B2
Application number	US-201816612103-A
Country	US
Kind code	B2
Filing date	May 11, 2018
Priority date	May 12, 2017
Publication date	Dec 6, 2022
Grant date	Dec 6, 2022

Abstract

An object of the present invention is to provide a method for efficiently obtaining a lactic acid bacterium that is made to contain a large amount of double-stranded RNA; and a lactic acid bacterium having a high double-stranded RNA content obtained by the method. The object is achieved by: (1) a method for producing a double-stranded RNA-containing lactic acid bacterium, including a step of culturing a lactic acid bacterium under at least one condition of an aeration condition and a low-temperature condition lower than an optimum temperature, thereby obtaining the double-stranded RNA-containing lactic acid bacterium; (2) a double-stranded RNA-containing lactic acid bacterium, in which the content of double-stranded RNA is 2.0 times or more as compared with the content of double-stranded RNA when a bacterium of the same strain is cultured for the same culture time under an optimum temperature and non-aeration condition; or the like.

First claim

The invention claimed is:

1. A double-stranded RNA-containing lactic acid bacterium,
wherein the content of double-stranded RNA of said lactic acid bacterium is 20 ng or more per mg of dry bacterial cells.

2. The double-stranded RNA-containing lactic acid bacterium of claim 1 ,
wherein the content of double-stranded RNA of said lactic acid bacterium is 50 ng or more per mg of dry bacterial cells.

3. The double-stranded RNA-containing lactic acid bacterium of claim 1 ,
wherein the content of double-stranded RNA of said lactic acid bacterium is 100 ng or more per mg of dry bacterial cells.

4. The double-stranded RNA-containing lactic acid bacterium of claim 1 ,
wherein the content of double-stranded RNA of said lactic acid bacterium is 200 ng or less per mg of dry bacterial cells.

5. The double-stranded RNA-containing lactic acid bacterium of claim 2 ,
wherein the content of double-stranded RNA of said lactic acid bacterium is 200 ng or less per mg of dry bacterial cells.

6. The double-stranded RNA-containing lactic acid bacterium of claim 3 ,
wherein the content of double-stranded RNA of said lactic acid bacterium is 200 ng or less per mg of dry bacterial cells.

Assignees

• Kikkoman Corp

Inventors

• Kawashima Tadaomi
• IKARI Naho
• Kawamoto Hiroshi

Classifications

• A23K10/18

  of live microorganisms · CPC title

• C12N2500/02

  Atmosphere, e.g. low oxygen conditions · CPC title

• C12N15/746

  for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus) · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• C12Q1/6834

  Enzymatic or biochemical coupling of nucleic acids to a solid phase · CPC title