Preparation and purification methods for mesenchymal stem cell derived secretome
US-2024091268-A1 · Mar 21, 2024 · US
US11517504B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11517504-B2 |
| Application number | US-202016777224-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 30, 2020 |
| Priority date | Jul 31, 2014 |
| Publication date | Dec 6, 2022 |
| Grant date | Dec 6, 2022 |
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A method of making a storage bag comprising the following steps performed in any order: (a) forming a composite tube having an inner surface comprising a higher melting polymer, an outer surface comprising a lower melting polymer, two ends, and a diameter; (b) flattening the tube in a direction perpendicular to the diameter; (c) forming a joint at one of the ends at a temperature between respective melting points of the higher melting polymer and the lower melting polymer; (d) providing a composite sheet having a first side comprising a higher melting polymer and a second side comprising a lower melting polymer; and (e) disposing the composite sheet over the joint such that the first side comprising a lower melting polymer engages the joint and forming a lap seam over the joint at a temperature between respective melting points of the higher melting polymer and the lower melting polymer, and a storage bag made by such method.
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What is claimed is: 1. A method of making a storage bag comprising the following steps performed in any order: (a) forming a composite tube having an inner surface comprising a higher melting polymer, an outer surface comprising a lower melting polymer, two ends, and a longitudinal length extending between the two ends; (b) flattening said tube along the longitudinal length of the tube; (c) forming a joint at one of said two ends at a temperature between respective melting points of said higher melting polymer and said lower melting polymer; (d) providing a composite sheet having a joint comprising a higher melting polymer and a second side comprising a lower melting polymer; (e) disposing said composite sheet over said joint such that said first side comprising a lower melting polymer engages said joint and forming a lap seam over said joint at a temperature between respective melting points of said higher melting polymer and said lower melting polymer. 2. A method of making a storage bag as defined in claim 1 wherein said storage bag is a cryopreservation bag. 3. A method of making a storage bag as defined in claim 1 further comprising performing steps (c) through (e) at the other of said two ends. 4. A method of making a storage bag as defined in claim 3 further comprising the steps of forming a handle in said one of said ends and attaching ports and a brace to said other of said two ends. 5. A method of making a storage bag as defined in claim 4 wherein the ports are attached before the composite tube is formed. 6. A method of making a storage bag as defined in claim 4 wherein the ports are attached after the composite tube is formed. 7. A method of making a storage bag as defined in claim 1 wherein step (e) further comprising disposing a composite sheet on a back side of said joint. 8. A method of making a storage bag as defined in claim 1 wherein step (e) comprises folding said composite sheet over said one of said ends to form lap seam over front and back sides of said joint. 9. A method of making a storage bag as defined in claim 1 wherein said higher melting polymer is a non-melt-processible polymer. 10. A method of making a storage bag as defined in claim 1 wherein said higher melting polymer is polytetrafluoroethylene. 11. A method of making a storage bag as defined in claim 1 wherein said higher melting polymer is polyimide. 12. A method of making a storage bag as defined in claim 1 wherein said lower melting polymer is a melt-processible polymer. 13. A method of making a storage bag as defined in claim 1 wherein said lower melting polymer is FEP. 14. A method of making a cryopreservation bag comprising the following steps performed in any order: (a) forming a composite tube having an inner surface comprising a non-melt-processible polymer, an outer surface comprising a melt-processible polymer, two ends, wherein the tube is divided into a first half and a second half, wherein each of the first half and the second half extends longitudinally between the two ends; (b) providing, on at least one of said two ends of said tube, an extension of said first half of said tube; (c) flattening said tube along a longitudinal length of the tube; (d) dividing said extension into at least three sections having edges; (e) folding said three sections such that said edges engage said second half of said tube to form a joint; (f) providing a composite sheet having a first side comprising a non-melt-processible polymer and a second side comprising a melt-processible polymer; (g) folding said composite sheet over said joint such that said first side comprising a melt-processible polymer engages said joint to form a lap seam over said joint.
Details of, or accessories for, sacks or bags · CPC title
characterised by the composition of the plastics material of the parts to be joined (welding bar compositions B29C65/125) · CPC title
of the parts to be joined, e.g. the joining process taking the temperature of the parts to be joined into account · CPC title
Flexible containers made from sheets or blanks, e.g. from flattened tubes · CPC title
covering both elements to be joined · CPC title
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