Detection of vitamins A and E by tandem mass spectrometry

Patent metadata

Field	Value
Publication number	US-11513130-B2
Application number	US-202017114276-A
Country	US
Kind code	B2
Filing date	Dec 7, 2020
Priority date	Sep 15, 2010
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

Methods are described for measuring the amount of one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample.

First claim

That which is claimed is:

1. A method for determining an amount of vitamin A and γ-tocopherol in a sample by tandem mass spectrometry, said method comprising:
a. adding one or more deuterated internal standards to the sample;
b. subjecting the sample to turbulent flow liquid chromatography (TFLC) to provide an extracted sample;
c. subjecting the extracted sample to high pressure liquid chromatography (HPLC) to provide a purified sample;
d. ionizing vitamin A and γ-tocopherol from the purified sample by positive ion mode to produce one or more vitamin A ions and γ-tocopherol ions detectable by tandem mass spectrometry; and
e. determining an amount of one or more of said Vitamin A ions and γ-tocopherol ions by tandem mass spectrometry;
wherein the amount of the one or more ions determined in step (e) is related to the amount of vitamin A and γ-tocopherol in the sample.

2. The method of claim 1 , wherein said HPLC and said ionization are conducted with on-line processing.

3. The method of claim 1 , wherein said ionization comprises ionization with an atmospheric pressure chemical ionization (APCI) source.

4. The method of claim 1 , wherein the sample is from a human patient suspected of having a deficiency or an excess of vitamin A.

5. The method of claim 1 , wherein the sample comprises serum or plasma.

6. The method of claim 1 , wherein the tandem mass spectrometry is conducted by multiple reaction monitoring, precursor ion scanning, or product ion scanning.

7. The method of claim 1 , the method further comprising determining an amount of one or more additional analytes simultaneously with the determination of the amount of vitamin A and γ-tocopherol.

8. The method of claim 7 , wherein the one or more additional analytes comprises one or more of α-tocopherol and β-tocopherol.

9. The method of claim 1 , wherein the one or more deuterated internal standards is d 5 -vitamin A.

10. The method of claim 1 , wherein the one or more deuterated internal standards is d 9 -α-tocopherol.

Assignees

• Quest Diagnostics Invest Inc
• Quest Diagnostics Invest Incoporated

Inventors

• Chan Sum
• Jiang Qibo

Classifications

• Y10T436/24

  Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry · CPC title

• H01J49/0045

  characterised by the fragmentation or other specific reaction · CPC title

• G01N33/48

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• G01N33/82 Primary

  involving vitamins {or their receptors} · CPC title