Composition for producing tagatose from fructose-6-phosphate and method of producing tagatose from fructose-6-phosphate using the same

Patent metadata

Field	Value
Publication number	US-11512301-B2
Application number	US-201816499138-A
Country	US
Kind code	B2
Filing date	Mar 30, 2018
Priority date	Mar 31, 2017
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

The present disclosure relates to a composition for producing tagatose, comprising fructose-6-phosphate-4-epimerase, and a method of producing tagatose using the same.

First claim

What is claimed is:

1. A method of producing tagatose, comprising:
a) producing tagatose-6-phosphate by contacting fructose-6-phosphate with tagatose-bisphosphate aldolase, a microorganism expressing the tagatose-bisphosphate aldolase, or a culture of the microorganism; and
b) producing tagatose by converting tagatose-6-phosphate produced in Step a) into tagatose.

2. The method of claim 1 , wherein the Step b) further comprises contacting the tagatose-6-phosphate produced in Step a) with tagatose-6-phosphate phosphatase, a microorganism expressing the tagatose-6-phosphate phosphatase, or a culture of the microorganism.

3. The method of claim 1 , wherein prior to producing the tagatose-6-phosphate, the method further comprises producing fructose-6-phosphate by converting glucose-6-phosphate into fructose-6-phosphate by contacting glucose-6-phosphate with glucose-6-phosphate-isomerase, a microorganism expressing the glucose-6-phosphate-isomerase, or a culture of the microorganism.

4. The method of claim 3 , wherein prior to producing the fructose-6-phosphate, the method further comprising comprises producing glucose-6-phosphate by converting glucose-1-phosphate into glucose-6-phosphate by contacting glucose-1-phosphate with phosphoglucomutase, a microorganism expressing the phosphoglucomutase, or a culture of the microorganism.

5. The method of claim 4 , wherein prior to producing the glucose-6-phosphate, the method further comprises producing glucose-1-phosphate by converting starch, maltodextrin, or sucrose into glucose-1-phosphate by contacting starch, maltodextrin, sucrose, or a combination thereof with (i) α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, or sucrose phosphorylase; (ii) a microorganism expressing the α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, or sucrose phosphorylase; or (iii) a culture of the microorganism.

6. The method of claim 3 , wherein prior to producing the fructose-6-phosphate, the method further comprises producing glucose-6-phosphate by converting glucose into glucose-6-phosphate by contacting glucose with glucokinase, a microorganism expressing the glucokinase, or a culture of the microorganism.

7. The method of claim 6 , wherein prior to producing the glucose-6-phosphate, the method further comprises producing glucose by converting starch, maltodextrin or sucrose into glucose by contacting starch, maltodextrin, sucrose, or a combination thereof with α-amylase, pullulanase, glucoamylase, sucrase, or isoamylase; a microorganism expressing the α-amylase, pullulanase, glucoamylase, sucrase, or isoamylase; or a culture of the microorganism.

8. The method of claim 1 , wherein the contacting is performed at pH 5.0 to 9.0, 40° C. to 80° C., and/or for 0.5 hours to 24 hours.

9. The method of claim 1 , wherein the tagatose-bisphosphate aldolase consists of an amino acid sequence of SEQ ID NO: 1, 3, 5, or 7.

10. A method of producing tagatose, comprising:
contacting (a) starch, maltodextrin, sucrose, or a combination thereof with (b) (i) tagatose-6-phosphate phosphatase, (ii) tagatose-bisphosphate aldolase, (iii) glucose-6-phosphate-isomerase, (iv) phosphoglucomutase or glucokinase, (v) phosphorylase, and (vi) one or more of α-amylase, pullulanase, isoamylase, glucoamylase, or sucrase; and (c) phosphate,
wherein the tagatose-bisphosphate aldolase has activity to convert fructose-6-phosphate into tagatose-6-phosphate.

11. The method of claim 2 , wherein the tagatose-6-phosphate phosphatase consists of the amino acid sequence of SEQ ID NO: 11.

Assignees

• Cj Cheiljedang Corp

Inventors

• Yang Sung Jae
• Cho Hyun Kug
• Lee Young Mi
• Kim Seong Bo

Classifications

• C12N9/90 Primary

  Isomerases (5.) · CPC title

• C12P19/24 Primary

  produced by the action of an isomerase, e.g. fructose · CPC title

• C12Y204/01025

  4-Alpha-glucanotransferase (2.4.1.25) · CPC title

• C12Y207/01144

  Tagatose-6-phosphate kinase (2.7.1.144) · CPC title

• C12N9/88 Primary

  Lyases (4.) · CPC title