Antimicrobial peptide Scyreprocin of Scylla paramamosain and method thereof

Patent metadata

Field	Value
Publication number	US-11512120-B2
Application number	US-202117230243-A
Country	US
Kind code	B2
Filing date	Apr 14, 2021
Priority date	Nov 21, 2018
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

The present disclosure discloses an antimicrobial peptide Scyreprocin of Scylla paramamosain and a method thereof, wherein an amino acid sequence of the antimicrobial peptide Scyreprocin comprises a sequence shown in SEQ ID NO 01, and the antimicrobial peptide is expressed and purified by using genetic engineering technology. The recombinant antimicrobial peptide Scyreprocin has advantages of wide antimicrobial spectrum, good antimicrobial effect, and rapid germicidal rate, shows great application significance, and has good application in preparation of antimicrobial agents. The recombinant antimicrobial peptide Scyreprocin has no cytotoxicity to mouse hepatocytes AML12, human liver cells L02, and can be safely used for medical treatment or can be used as a feed composition.

First claim

What is claimed is:

1. A method for preparing an antimicrobial composition to be used to inhibit growth of microorganisms, wherein the method comprises recombination expression and purification of an antimicrobial peptide Scyreprocin of Scylla paramamosain with a His tag, wherein an amino acid sequence of the antimicrobial peptide Scyreprocin consists of SEQ ID NO: 01, and wherein the recombination expression and purification comprises:
cloning an open reading frame of the antimicrobial peptide Scyreprocin of Scylla paramamosain and constructing into a vector pET28a to obtain a recombinant vector; and
transferring the recombinant vector into Escherichia coli BL21 to obtain a recombinant expression strain configured to express the antimicrobial peptide Scyreprocin of Scylla paramamosain with a protein purification solution.

2. The method according to claim 1 , wherein the antimicrobial composition is a feed composition.

3. The method according to claim 1 , wherein the antimicrobial composition is an anti-mildew and antiseptic composition.

Assignees

• Univ Xiamen

Inventors

• Wang Kejian
• Yang Ying
• Chen Fangyi
• Chen Yanchao
• Chen Huiyun
• Peng Hui

Classifications

• A23V2250/55

  Peptide, protein hydrolysate · CPC title

• C07K14/43509 Primary

  from crustaceans · CPC title

• A23K50/75

  for poultry · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K38/17

  from animals; from humans {(enzyme inhibitors A61K38/005)} · CPC title