Conductive biomimetic skin scaffold material with self-repairing function and a method of preparing the same

Patent metadata

Field	Value
Publication number	US-11511018-B2
Application number	US-202117203689-A
Country	US
Kind code	B2
Filing date	Mar 16, 2021
Priority date	Jul 23, 2020
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

A method for preparing a conductive biomimetic skin scaffold material with self-repairing function includes the following steps: adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to a homogeneous dispersion of acidified carbon nanotubes, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), and gelatin to cross-link to obtain a conductive composite colloid; and injecting the conductive composite colloid into a mold, aging at −4-4° C. for 12-24 hours, and then soaking in a phosphate-buffered saline (PBS) solution with a pH of 7.0-7.4 for 12-24 hours to obtain the conductive biomimetic skin scaffold material.

First claim

What is claimed is:

1. A method for preparing a conductive biomimetic skin scaffold material with self-repairing function, comprising the following steps:
adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to a homogeneous dispersion of acidified carbon nanotubes, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), and gelatin to cross-link to obtain a conductive composite colloid; and
injecting the conductive composite colloid into a mold, aging at −4-4° C. for 12-24 hours, and then soaking in a phosphate-buffered saline (PBS) solution with a pH of 7.0-7.4 for 12-24 hours to obtain the conductive biomimetic skin scaffold material.

2. The method according to claim 1 , further comprising:
dispersing 1.0-5.0 mL of a PEDOT:PSS solution and 1.0-5.0 mL of a 0.05-1.0 g/mL acidified carbon nanotube solution in 10.0-50.0 mL of water to obtain a mixture;
adding 1.0-10.0 g of gelatin to the mixture to evenly disperse the mixture;
adding 1.0-5.0 mL of a 0.02-0.5 g/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride aqueous solution to the mixture; and
stirring the mixture at 30-60° C. to obtain the conductive composite colloid.

3. The method according to claim 1 , further comprising:
(1) adding 10.0-50.0 mL water, 1.0-5.0 mL a PEDOT:PSS solution, and 1.0-5.0 mL of a 0.05-1.0 g/mL acidified carbon nanotube solution to a reactor to form a mixture, and ultrasonicating the mixture at 50-100 W, 40 kHz, for 30-120 minutes;
(2) adding 5.0-20.0 g of gelatin to the mixture, stirring the mixture at 30-60° C. for 30-120 minutes to evenly disperse the mixture; and
(3) dissolving 0.1-0.5 g of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) powder in 1.0-5.0 mL water to obtain a 0.02-0.5 g/mL EDC solution; adding 0.1-2.0 mL of the EDC solution slowly to the mixture of step (2), stirring for 30-120 minutes to at 30-60° C. to obtain the conductive composite colloid.

4. The method of claim 1 , wherein the gelatin is derived from a fetal bovine acellular dermal matrix.

5. The method of claim 1 , further comprising:
reacting 1.0-5.0 g of multi-walled carbon nanotubes, 50.0-250.0 g of 98% concentrated H 2 SO 4 , and 20.0-100.0 g of 65-68% HNO 3 at 50-100° C. for 5-15 hours to obtain an acidified carbon nanotube solution.

6. The method of claim 5 , further comprising:
centrifugating the acidified carbon nanotube solution at a speed of 1000 to 5000 rpm for 10-60 minutes, and filtering;
adding 100.0-500.0 mL of water, centrifugating at 5000-8000 rpm for 10-60 minutes, and filtering;
adding 100.0-500.0 mL of water, centrifugating at 8000-10000 rpm for 10-60 minutes; and
freeze-drying to obtain the acidified carbon nanotubes.

Assignees

• Wang Xuechuan
• Zheng Manhui
• Liu Xinhua
• Yue Ouyang
• Hou Mengdi
• Wang Youyou
• Univ Shaanxi Science & Tech

Inventors

• Wang Xuechuan
• Zheng Manhui
• Liu Xinhua
• Yue Ouyang
• Hou Mengdi
• Wang Youyou

Classifications

• A61L27/26

  Mixtures of macromolecular compounds · CPC title

• A61L27/443

  with carbon fillers · CPC title

• B29K2507/04

  Carbon · CPC title

• A61L27/48

  with macromolecular fillers · CPC title

• B29K2105/167

  Nanotubes · CPC title