Lipolytic enzyme for use in baking

Patent metadata

Field	Value
Publication number	US-11510413-B2
Application number	US-202117337613-A
Country	US
Kind code	B2
Filing date	Jun 3, 2021
Priority date	Feb 20, 2017
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

The present invention relates to a polypeptide having lipolytic enzyme activity, selected from the group consisting of: (a) a polypeptide having at least 65% sequence identity to amino acids 21 to 309 of SEQ ID NO: 1; (b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with the polypeptide coding sequence of SEQ ID NO: 2; (c) a polypeptide encoded by a polynucleotide having at least 65% sequence identity to the polypeptide coding sequence of SEQ ID NO: 2; and (d) a fragment of the polypeptide of (a), (b) or (c) that has lipolytic enzyme activity.

First claim

The invention claimed is:

1. A recombinant microbial host cell transformed with a polynucleotide encoding a polypeptide having lipolytic activity,
wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, and
wherein the polypeptide comprises an amino acid sequence having at least 80% sequence identity to the sequence of amino acids 21 to 309 of SEQ ID NO: 1.

2. A method of producing a polypeptide having lipolytic activity, comprising cultivating the recombinant host cell of claim 1 under conditions conducive for production of the polypeptide.

3. The method of claim 2 , further comprising recovering the polypeptide.

4. The method of claim 2 , wherein the recombinant host cell is a Gram-positive or Gram-negative bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus, Streptomyces, Campylobacter, Escherichia coli, Flavobacterium, Fusobacterium, Helicobacter, Ilyobacter, Neisseria, Pseudomonas, Salmonella , and Ureaplasma.

5. The method of claim 4 , wherein the recombinant host cell is a Bacillus cell selected from the group consisting of Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis , and Bacillus thuringiensis cells.

6. The method of claim 2 , wherein the recombinant host cell is a yeast cell selected from the group consisting of Candida, Hansenula, Kluyveromyces, Pichia, Saccharomyces, Schizosaccharomyces , and Yarrowia cells.

7. The method of claim 2 , wherein the recombinant host cell is a filamentous fungal cell selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes , and Trichoderma cells.

8. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence G-H-S-L-G (SEQ ID NO: 5).

9. The method of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 85% sequence identity to the sequence of amino acids 21 to 309 of SEQ ID NO: 1.

10. The method of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 90% sequence identity to the sequence of amino acids 21 to 309 of SEQ ID NO: 1.

11. The method of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 21 to 309 of SEQ ID NO: 1.

12. The method of claim 2 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the sequence of amino acids 21 to 309 of SEQ ID NO: 1.

13. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence of amino acids 21 to 309 of SEQ ID NO: 1.

14. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence of amino acids 21 to 309 of SEQ ID NO: 6.

15. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence of amino acids 21 to 309 of SEQ ID NO: 7.

16. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence of amino acids 21 to 309 of SEQ ID NO: 8.

17. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence of amino acids 21 to 309 of SEQ ID NO: 9.

18. The method of claim 2 , wherein the polypeptide is a fragment of the sequence of amino acids 21 to 309 of SEQ ID NO: 1, wherein the fragment has lipase activity.

19. The method of claim 2 , wherein the polypeptide has lipase and phospholipase activity.

20. The recombinant host cell of claim 1 , wherein the one or more control sequences is heterologous to the polynucleotide.

Assignees

• Novozymes As
• Puratos Nv/Sa

Inventors

• Oestdal Henrik
• Landvik Sara Maria
• Olinski Robert Piotr
• Agache Evelien
• Van Winckel Bruno
• Arnaut Filip

Classifications

• A21D8/042 Primary

  with enzymes · CPC title

• A21D10/002

  Dough mixes; Baking or bread improvers; Premixes · CPC title

• C12Y301/01

  Carboxylic ester hydrolases (3.1.1) · CPC title

• A21D10/00

  Batters, dough or mixtures before baking · CPC title

• C12N9/20

  Triglyceride splitting, e.g. by means of lipase · CPC title