Illumination systems and devices for fourier ptychographic imaging
US-2017371141-A1 · Dec 28, 2017 · US
US11506485B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11506485-B2 |
| Application number | US-201916664734-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 25, 2019 |
| Priority date | Apr 26, 2017 |
| Publication date | Nov 22, 2022 |
| Grant date | Nov 22, 2022 |
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An optical system includes an illumination module configured to illuminate a sample object with at least one angle-variable illumination geometry. The optical system includes an imaging optical unit configured to produce an imaged representation of the sample object that is illuminated with the at least one angle-variable illumination geometry on a detector. The optical system includes the detector, which is configured to capture at least one image of the sample object based on the imaged representation. The optical system includes a controller configured to determine a result image based on a transfer function and the at least one image. A method includes illuminating a sample object with at least one angle-variable illumination geometry, imaging the sample object on a detector, based on the imaged representation, capturing at least one image of the sample object, and, based on a transfer function and the at least one image, determining a result image.
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The invention claimed is: 1. An optical system comprising: an illumination module configured to illuminate a sample object with at least one pair of angle-variable illumination geometries corresponding to different illumination directions, thus implementing an illumination structured in angle space; an imaging optical unit configured to produce an imaged representation of the sample object that is illuminated with the at least one pair of angle-variable illumination geometries on a detector; the detector, wherein the detector is configured to capture at least one pair of real-space images of the sample object based on the imaged representation; and a controller configured to: determine a transfer function based on the at least one pair of angle-variable illumination geometries; determine a pairwise difference for each pair of the at least one pair of real-space images; determine a spectral decomposition of the pairwise difference; and determine a real-space result image based on the transfer function and the spectral decomposition. 2. The optical system of claim 1 , wherein the illumination module and the detector are arranged in reflected light geometry. 3. The optical system of claim 1 , wherein the illumination module is configured to illuminate the sample object in dark field geometry. 4. The optical system of claim 1 , wherein: the controller is configured to detect anomalies in the real-space result image and the anomalies comprise extreme values in contrast of the real-space result image. 5. The optical system of claim 1 , wherein: the controller is configured to detect anomalies in the real-space result image and the anomalies comprise deviations from a real-space pattern of the sample object. 6. The optical system of claim 5 , wherein the controller is configured to determine the deviations from the real-space pattern of the sample object based on at least one of the following techniques: Fourier space filtering, autocorrelation, and deviations with respect to a reference image of the real-space pattern. 7. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; a lower threshold is defined based on 5% of a maximum value of all absolute values of the transfer function for spatial frequencies between —N A and N A ; and for spatial frequencies between —N A and N A , each absolute value of the transfer function is greater than the lower threshold. 8. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; a lower threshold is defined based on 2% of a maximum value of all absolute values of the transfer function for spatial frequencies between —N A and N A ; and for spatial frequencies between —N A and N A , each absolute value of the transfer function is greater than the lower threshold. 9. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; a lower threshold is defined based on 0.5% of a maximum value of all absolute values of the transfer function for spatial frequencies between — 2N A and 2N A ; and for spatial frequencies between — 2N A and 2N A , each absolute value of the transfer function is greater than the lower threshold. 10. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A and for spatial frequencies between —N A and N A , the transfer function has no local extreme values. 11. The optical system of claim 1 , wherein the transfer function is a step function. 12. The optical system of claim 1 , wherein the transfer function is one of a monotonously increasing function and a monotonously decreasing function. 13. The optical system of claim 12 , wherein the transfer function is at least one of a linear function and a sigmoid function. 14. The optical system of claim 1 , wherein the transfer function has an axis of symmetry that corresponds to an axis of symmetry of the at least one pair of angle-variable illumination geometries. 15. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; an upper threshold is defined based on 5% of a maximum value of all absolute values of the transfer function for spatial frequencies between —N A and N A ; and for spatial frequencies greater than 2N A and for spatial frequencies less than — 2N A , each absolute value of the transfer function is less than the upper threshold. 16. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; an upper threshold is defined based on 2% of a maximum value of all absolute values of the transfer function for spatial frequencies between — 2N A and 2N A ; and for spatial frequencies greater than 2N A and for spatial frequencies less than — 2N A , each absolute value of the transfer function is less than the upper threshold. 17. The optical system of claim 1 , wherein: the imaging optical unit is characterized by an aperture size, N A ; an upper threshold is defined based on 0.5% of a maximum value of all absolute values of the transfer function for spatial frequencies between —N A and N A ; and for spatial frequencies greater than N A and for spatial frequencies less than —N A , each absolute value of the transfer function is less than the upper threshold. 18. The optical system of claim 1 , wherein the controller is configured to determine the real-space result image based on a Tikhonov regularization with inverse Fourier transform. 19. A method comprising: illuminating a sample object with at least one pair of angle-variable illumination geometries corresponding to different illumination directions, thus implementing an illumination structured in angle space; producing an imaged representation of the sample object illuminated with the at least one pair of angle-variable illumination geometries on a detector; based on the imaged representation, capturing at least one pair of real-space images of the sample object; determining a transfer function based on the at least one pair of angle-variable illumination geometries; a pairwise difference of the at least one pair of real-space images; determining a spectral decomposition of the pairwise difference; and based on the transfer function and the spectral decomposition, determining a real-space result image. 20. The optical system of claim 1 , wherein the transfer function designates a transfer function other than an object transfer function or an optics transfer function. 21. The optical system of claim 1 , wherein the controller is configured to: determine a ratio of (i) a difference of each pair of the at least one pair of real-space images to (ii) a sum of the two images of the respective pair; and determine the real-space result image based on the transfer function and the ratio.
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