Process for transfer of a method from a starting system to a target system in liquid chromatography

Patent metadata

Field	Value
Publication number	US-11504647-B2
Application number	US-201615229553-A
Country	US
Kind code	B2
Filing date	Aug 5, 2016
Priority date	Aug 5, 2015
Publication date	Nov 22, 2022
Grant date	Nov 22, 2022

Abstract

A process for transferring a method from a starting system to a target system in liquid chromatography, in particular in high performance liquid chromatography, is described. A first chromatogram of the method carried out on the starting system is available or determined. The method developed for the starting system is carried out on the target system without any change in its physical parameters, and a second chromatogram is thereby determined. The two chromatograms of the starting system and the target system are compared, and measures for adjusting the physical system parameters of the target system are derived from the deviations.

First claim

What is claimed is:

1. A process for transfer of a method from a starting system to a target system in liquid chromatography, the process comprising:
(a) providing a first chromatogram of the method carried out on the starting system;
(b) performing the method of the starting system on the target system without any change in physical parameters and obtaining a second chromatogram;
(c) comparing the first chromatogram of the starting system and the second chromatogram of the target system to determine a deviation, and
(d) calculating a change in at least one of the physical parameters of the target system based on the deviation,
(e) performing the method developed for the starting system on the target system with the calculated change in the at least one physical parameter based on the deviation and obtaining a third chromatogram;
(f) comparing the first chromatogram of the starting system and the third chromatogram of the target system to determine a successive deviation,
(g) iteratively adjusting the at least one physical parameter of the target system derived from the successive deviation, and
(h) wherein comparing of the two chromatograms of the starting system and the target system, further comprises:
detecting corresponding peaks on the first chromatogram and the second chromatogram with a computer; and
calculating a displacement vector with the computer, in which the displacement vector is a distance over time of the detected corresponding peaks.

2. The process according to claim 1 , in which the deviation comprises a time shift for a peak in the second chromatogram compared to the first chromatogram, in which the physical parameters comprise a gradient delay volume, the process further comprising:
calculating a change in the gradient delay volume based on the time shift.

3. The process according to claim 2 , in which the physical parameters further comprise an injection time, the process further comprising:
calculating a change in the injection time based on the time shift.

4. The process according to claim 1 , in which the deviation comprises a time shift for a peak in the second chromatogram compared to the first chromatogram, in which the physical parameters comprise an injection time, the process further comprising:
calculating a change in the injection time based on the time shift.

5. The process according to claim 1 , in which the deviation comprises a time change between two peaks in the second chromatogram compared to a time change between two corresponding peaks in a first chromatogram, in which the physical parameters comprise a type of thermostatic regulation of a target column oven or a target column temperature, the process further comprising:
calculating a change in the type of thermostatic regulation of the target column oven or the target column temperature based on the deviation.

6. The process according to claim 5 , in which the time change between two peaks in the second chromatogram compared to the time change between two corresponding peaks in the first chromatogram comprises either a compression or a spreading.

7. The process according to claim 1 , in which the deviation comprises a time change between two peaks in the second chromatogram compared to a time change between two corresponding peaks in a first chromatogram, in which the physical parameters comprise a gradient slope, the process further comprising:
calculating a change in the gradient slope based on the deviation.

8. The process according to claim 1 further comprising:
varying a gradient delay volume to adjust a sharpness of a gradient and a peak sharpness.

9. The process according to claim 8 , in which the deviation comprises a time shift for a peak in the second chromatogram compared to the first chromatogram, in which the physical parameters comprise a gradient delay volume and an injection time, the process further comprising:
after adjusting the peak sharpness, calculating a change in the gradient delay volume or the injection time based on the time shift.

10. The process according to claim 1 , in that the physical parameter is derived from the displacement vector.

11. A process for transfer of a method from a starting system to a target system in liquid chromatography, the process comprising:
(a) providing a first chromatogram of the method carried out on the starting system;
(b) performing the method of the starting system on the target system without any change in physical parameters and obtaining a second chromatogram;
(c) comparing the first chromatogram of the starting system and the second chromatogram of the target system,
(d) calculating a change in at least one of the physical parameters of the target system,
(e) performing the method developed for the starting system on the target system with the calculated change in the at least one physical parameter and obtaining a third chromatogram; and
(f) comparing the first chromatogram of the starting system and the third chromatogram of the target system, including detecting corresponding peaks on the first chromatogram and the second chromatogram with a computer, and calculating a displacement vector with the computer, in which the displacement vector is a difference in height of the detected corresponding peaks.

12. The process according to claim 11 , in which comparing the first chromatogram and the second chromatogram comprises determining a time shift for a peak in the second chromatogram compared to the first chromatogram, in which the physical parameters comprise a gradient delay volume, the process further comprising calculating a change in the gradient delay volume based on the time shift.

13. The process according to claim 12 , in which the physical parameters further comprise an injection time, the process further comprising:
calculating a change in the injection time based on the time shift.

14. The process according to claim 13 , in which comparing the first chromatogram and the second chromatogram comprises determining a time shift for a peak in the second chromatogram compared to the first chromatogram, in which the physical parameters comprise an injection time, the process further comprising calculating a change in the injection time based on the time shift.

15. The process according to claim 11 , in which comparing the first chromatogram and the second chromatogram comprises determining a time change between two peaks in the second chromatogram compared to a time change between two corresponding peaks in the first chromatogram, in which the physical parameters comprise a type of thermostatic regulation of a target column oven or a target column temperature, the process further comprising:
calculating a change in the type of thermostatic regulation of the target column oven or the target column temperature based on the deviation.

16. The process according to claim 15 , in which the time change between two peaks in the second chromatogram compared to the time change between two corresponding peaks in the first chromatogram comprises either a compression or a spreading.

17. The process according to claim 11 , in which comparing the first chromatogram and the second chromatogram comprises determining a time change between two peaks in the second chromatogram compared to a time change between two corresponding peaks in the first chromatogram, in which the physical parameters comprise a gradient slope, the process further comprising calculating a change in the gradient slope based on the deviation.

18. The process according to claim 11 further comprising:
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Assignees

• Dionex Softron Gmbh

Inventors

• Franz Holger
• Wachinger Thomas
• Schmidt Christian

Classifications

• B01D15/166 Primary

  Fluid composition conditioning, e.g. gradient · CPC title

• B01D15/161

  Temperature conditioning · CPC title

• G01N30/86

  Signal analysis · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title

• B01D15/14

  relating to the introduction of the feed to the apparatus · CPC title