Two-phase electroextraction from moving phases

Patent metadata

Field	Value
Publication number	US-11504645-B2
Application number	US-201414898813-A
Country	US
Kind code	B2
Filing date	Jun 19, 2014
Priority date	Jun 19, 2013
Publication date	Nov 22, 2022
Grant date	Nov 22, 2022

Abstract

The present invention relates to a process for the electro extraction of molecules from a moving fluid donor phase into an acceptor phase, comprising the steps of: providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; and providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode.

First claim

The invention claimed is:

1. A process for an electroextraction of organic compounds from a moving fluid donor phase into an acceptor phase, the process comprising:
providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode;
providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode;
providing a supporting or confining phase guide pattern having a sharp edge to keep a defined interface between the donor phase and the acceptor phase, wherein the sharp edge is shaped such that advancement of a fluid over the sharp edge requires a change of a principal radii of a fluid-fluid meniscus, leading to a higher pressure drop over the fluid-fluid meniscus thus representing a pressure barrier; and
applying an electrical field between the first electrode and the second electrode, wherein the electrical field has a current between 0.01 μA and 100 μA for an interface length of 8 mm;
wherein one of the donor phase and the acceptor phase is an organic phase and an other of the donor phase and the acceptor phase is an aqueous phase; and
wherein the acceptor phase is moving at a second flow velocity which is lower than the first flow velocity.

2. The process according to claim 1 , wherein the acceptor phase is moving co-directionally with the donor phase.

3. The process according to claim 1 , wherein the donor phase and/or the acceptor phase remain immiscible during the process.

4. The process according to claim 1 , wherein the electrical field is applied sufficiently high and in a sufficiently long period of time to allow at least part of the organic compounds to migrate from the donor phase to the acceptor phase, or to the defined interface between the donor phase and the acceptor phase.

5. The process according to claim 1 , wherein a further acceptor phase is present at an opposite side of the acceptor phase and in direct contact with the donor phase, and wherein the first electrode is arranged in the further acceptor phase, and wherein the electrically conductive contact with the donor phase occurs through the further acceptor phase.

6. The process according to claim 1 , further comprising:
removing the acceptor phase comprising migrated analytes; and
subjecting the removed acceptor phase to a further separation and/or analysis process.

7. The process according to claim 6 , wherein the further separation and/or analytical process comprises one or more of liquid chromatography (LC), reversed phase (RP) LC, normal phase (NP) LC, thin layer chromatography (TLC), capillary electrophoresis (CE), nuclear magnetic resonance (NMR), mass spectrometry (MS) gas chromatography/mass spectrometry (GC/MS), GC-MS/MS, nanoelectrospray-Direct-Infusion-MS, ultraviolet/visible light (UV/VIS), nano LC, high-performance liquid chromatography (HPLC), ultra-high performance LC (UPLC); RP-(UV/VIS)-EV-NP-MS, RP-(UV/VIS)-EV-NMR, NP-(UV/VIS)-EV-CE-MS/RP-(UV/VIS)-EV-CE-MS, RP(UV/VIS)-EV-TLC/NP-(UV/VIS)-EV-TLC, EV-nano LC, and CE-EV.

8. The process according to claim 1 , wherein the donor phase is a result of a previous separation and/or analysis process.

9. The process according to claim 1 , wherein the organic compounds are selected from pharmaceuticals, drugs, coloring agents, food additives, metabolites, nucleotides (such as DNA or RNA), proteins, peptides, amino acids, carbohydrates, lipids, polysaccharide fatty acids or phospholipids.

Assignees

• Univ Leiden

Inventors

• Schoonen Jan-Willem
• Lindenburg Peter
• Van Duinen Vincent
• Oedit Amar
• Vulto Paul
• Hankemeier Thomas

Classifications

• G01N2001/4072

  membraneless transfer of a component between two parallel laminar flows of fluid · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L2200/0636

  Focussing flows, e.g. to laminate flows · CPC title

• B01D11/0419 Primary

  in combination with an electric or magnetic field or with vibrations · CPC title

• B01D11/0496

  by extraction in microfluidic devices · CPC title