Pulp regeneration compositions and methods of forming and using the same

Patent metadata

Field	Value
Publication number	US-11504303-B2
Application number	US-202117557483-A
Country	US
Kind code	B2
Filing date	Dec 21, 2021
Priority date	Dec 1, 2016
Publication date	Nov 22, 2022
Grant date	Nov 22, 2022

Abstract

A dental tissue regenerative composition. The composition includes a combination of (1) human dental pulp stem cells and (2) at least one of human umbilical vein endothelial cells or vascular endothelial growth factor. The combination is encapsulated in a light-activated gelatin methacrylate hydrogel.

First claim

What is claimed is:

1. A dental tissue regenerative composition comprising:
a material at least partly obtained from an extracted tooth; and
a light-activated gelatin methacrylate hydrogel encapsulating the material.

2. The composition of claim 1 , further comprising a photo-initiator.

3. The composition of claim 2 , wherein the photo-initiator is present at a concentration of about 0.05% to about 0.5% (w/v).

4. The composition of claim 1 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.

5. The composition of claim 1 , wherein the gelatin methacrylate hydrogel is present at a concentration of about 3% to about 5% (w/v).

6. The composition of claim 1 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.

7. The composition of claim 6 , wherein the material further includes at least one of umbilical vein endothelial cells or vascular endothelial growth factor.

8. The composition of claim 6 , wherein the dental pulp stem cells have been isolated from dental pulp.

9. The composition of claim 8 , wherein the dental pulp is from a mature tooth.

10. The composition of claim 1 , wherein the composition is configured to be used for pulpal revascularization, to replace infected pulpal tissues in pulpotomy, as a temporary intra canal medicament, or for regenerating pulp-like tissues in tooth root segments.

11. A method of regenerating pulp-like tissues in a tooth root segment of a tooth, the method comprising:
providing a composition comprising
a gelatin methacrylate hydrogel,
a photo-initiator, and
a material at least partly obtained from an extracted tooth, the gelatin methacrylate hydrogel and the material having been cross-linked by exposure to a UV or visible light; and

injecting the composition into the tooth root segment.

12. The method of claim 11 , wherein the photo-initiator is present in the composition at a concentration of about 0.05% to about 0.5% (w/v).

13. The method of claim 11 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.

14. The method of claim 11 , wherein the gelatin methacrylate hydrogel is present in the composition at a concentration of about 3% to about 5% (w/v).

15. The method of claim 11 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.

16. The method of claim 15 , wherein the material further comprises at least one of umbilical vein endothelial cells or vascular endothelial growth factor.

17. The method of claim 15 , wherein the dental pulp stem cells have been isolated from dental pulp.

18. The method of claim 17 , wherein the dental pulp is from a mature tooth.

19. A method of forming a dental tissue regenerative composition, the method comprising:
combining a material at least partly obtained from an extracted tooth, a gelatin methacrylate hydrogel, and a photo-initiator; and
cross-linking the gelatin methacrylate hydrogel with the material by exposure to a UV or visible light.

20. The method of claim 19 , wherein the photo-initiator is present at a concentration of about 0.05% to about 0.5% (w/v).

21. The method of claim 19 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.

22. The method of claim 19 , wherein the gelatin methacrylate hydrogel is present at a concentration of about 3% to about 5% (w/v).

23. The method of claim 19 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.

24. The method of claim 23 , wherein the material further comprises at least one of umbilical vein endothelial cells or vascular endothelial growth factor.

25. The method of claim 23 , wherein the dental pulp stem cells have been isolated from dental pulp.

26. The method of claim 25 , wherein the dental pulp is from a mature tooth.

27. The method of claim 19 , wherein the composition is configured to be used for pulpal revascularization, to replace infected pulpal tissues in pulpotomy, as a temporary intra canal medicament or for regenerating pulp-like tissues in tooth root segments.

Assignees

• Tufts College
• Brigham & Womens Hospital Inc

Inventors

• Yelick Pamela C
• Khademhosseini Ali

Classifications

• A61K35/51 Primary

  Umbilical cord; Umbilical cord blood; Umbilical stem cells · CPC title

• A61C5/40

  Implements for surgical treatment of the roots or nerves of the teeth; Nerve needles; Methods or instruments for medication of the roots · CPC title

• A61K6/69

  Medicaments · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• A61L27/3808

  Endothelial cells · CPC title