Coated substrate for biological reaction systems

Patent metadata

Field	Value
Publication number	US-11493526-B2
Application number	US-202016852830-A
Country	US
Kind code	B2
Filing date	Apr 20, 2020
Priority date	Mar 16, 2012
Publication date	Nov 8, 2022
Grant date	Nov 8, 2022

Abstract

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

First claim

What is claimed is:

1. An apparatus for biological reactions, the apparatus comprising:
a substrate comprising a substrate surface;
a plurality of reaction sites within the substrate, wherein each reaction site comprises a reaction site surface; and
a coating material, wherein the coating material coats the substrate surface and each reaction site surface, the coated substrate surface has a water contact angle of 60-100 degrees, the coated substrate surface has an advancing contact angle of 70-85 degrees, and each of the coated reaction site surfaces is configured to load the reaction sites with a sample for undergoing a biological reaction.

2. The apparatus of claim 1 , wherein the coating material configures the substrate surface and the each of the reaction site surfaces to have the same hydrophilicity.

3. The apparatus of claim 1 , wherein the difference between the advancing water contact angle and the receding contact angle of the substrate surface is 0 to 30 degrees.

4. The apparatus of claim 1 , wherein the coating material is hexamethyldisilazane (HMDS).

5. The apparatus of claim 1 , wherein the substrate surface and each reaction site surface are coated by a vapor deposition process.

6. The apparatus of claim 1 , wherein the sample volume loaded into the plurality of reaction sites is at most 1 nanoliter.

7. The apparatus of claim 1 , wherein the substrate is composed of one material of the following: silicon, silicon oxide, glass, and plastic.

8. The apparatus of claim 1 , wherein the plurality of reaction sites are through-holes.

9. The apparatus of claim 1 , wherein the biological reaction is an amplification reaction.

10. The apparatus of claim 1 , wherein the coating material results in a hydrophilicity to generate a sufficient surface tension force to substantially confine the sample within each loaded reaction site.

11. The apparatus of claim 1 , wherein capillary action determines a volume of sample loaded into each reaction site, wherein the amount of capillary action is based on a dimension of each reaction site.

12. An instrument, comprising:
a detection system;
control system configured to control the functions of the detection system;
a sample support device including substrate comprising a substrate surface and a plurality of reaction sites disposed along the substrate surface, each reaction site comprising a reaction site surface;
a coating material, wherein:
the coating material coats the substrate surface, to provide a coated substrate surface,
the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites,
the coated substrate surface has a water contact angle of 60-100 degrees, and
the coated substrate surface has an advancing contact angle of 70-85 degrees.

13. The instrument of claim 12 , wherein
each of the coated reaction site surfaces is configured to load the reaction sites with a sample; and
each sample is configured to undergo a biological reaction within the reaction site.

14. The instrument of claim 12 , wherein the instruments is a polymerase chain reaction instrument.

Assignees

• Life Technologies Corp

Inventors

• Ghenciu Eliodor
• Straub Theodore E
• Foster Evan W
• Pallas Michael C

Classifications

• B01L2300/0893

  having a very large number of wells, microfabricated wells · CPC title

• B01L2200/12

  Specific details about manufacturing devices · CPC title

• B01J2219/00317

  Microwell devices, i.e. having large numbers of wells · CPC title

• B01L2300/165

  Specific details about hydrophobic, oleophobic surfaces · CPC title

• B01J2219/00637

  by coating it with another layer · CPC title