Tomato plant producing fruits with anthocyanins

Patent metadata

Field	Value
Publication number	US-11492634-B2
Application number	US-201815937160-A
Country	US
Kind code	B2
Filing date	Mar 27, 2018
Priority date	Oct 30, 2015
Publication date	Nov 8, 2022
Grant date	Nov 8, 2022

Abstract

The present invention relates to a tomato plant which carries at least one QTL in its genome that leads to its fruits comprising higher levels of anthocyanins when compared to fruits produced by a tomato plant not carrying said QTL in its genome, wherein said fruits are not purple at the red-ripe harvest stage. A tomato plant of the invention may also comprise all QTLs, each either in homozygous or heterozygous form. The invention further relates to progeny of the plant, propagation material for the plant and to markers for identifying the QTLs and their use.

First claim

What is claimed is:

1. A tomato plant which carries at least two QTLs in its genome that leads to producing a fruit comprising higher levels of anthocyanins when compared a fruit produced by a tomato plant not carrying said QTLs in its genome, wherein said fruit produced by the tomato plant which carries at least two QTLs are not purple at the red-ripe harvest stage,
wherein the two QTLs comprise a first QTL1 and a second QTL3,
wherein QTL1 comprises a region of chromosome 10 from the genome of a tomato plant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42470,
wherein QTL1 comprises a region including and between SEQ ID NO: 1 and the end of chromosome 10,
wherein QTL3 comprises a region of chromosome 7 from the genome of a tomato plant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42470, and
wherein QTL3 comprises a region including and between SEQ ID NO: 13 and SEQ ID NO: 14.

2. The tomato plant of claim 1 ,
wherein the ‘C’ at position 61 in SEQ ID NO: 1 corresponds to the physical position 63,102,099 on the public tomato genome and the end of said chromosome corresponds to the physical position 65,527,505 on the public tomato genome.

3. The tomato plant of claim 1 ,
wherein the presence of the QTL1 on chromosome 10 is identified by a marker having SEQ ID NO: 2,
wherein the ‘C’ at position 139 in SEQ ID NO: 2 corresponds to the physical position 65,134,950 on the public tomato genome and/or by a marker having SEQ ID NO: 3, and
wherein the ‘ T’ at position 141 in SEQ ID NO: 3 corresponds to the physical position 65,133,628 on the public tomato genome.

4. The tomato plant of claim 1 ,
wherein the at position 61 in SEQ ID NO: 13 corresponds to the physical position 59,721,395 on the public tomato genome and ‘G’ at position 61 in SEQ ID NO: 14 corresponds to the physical position 62,964,169 on the public tomato genome.

5. The tomato plant of claim 4 , wherein the presence of the QTL3 on chromosome 7 is identified by one or more of the following markers: SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19 or SEQ ID NO: 20 and wherein
a marker having SEQ ID NO: 15 wherein the ‘C’ at position 61 in SEQ ID NO: 15 corresponds to the physical position 61,333,917 on the public tomato genome;
a marker having SEQ ID NO: 16, wherein the ‘C’ at position 61 in SEQ ID NO: 16 corresponds to the physical position 60,557,208 the public tomato genome;
a marker having SEQ ID NO: 17, wherein the ‘T’ at position 79 in SEQ ID NO: 17 corresponds to the physical position 60,747,126 on the public tomato genome;
a marker having SEQ ID NO: 18, wherein the ‘G’ at position 79 in SEQ ID NO: 18 corresponds to the physical position 61,000,734 on the public tomato genome;
a marker having SEQ ID NO: 19, wherein the ‘T’ at position 79 in SEQ ID NO: 19 corresponds to the physical position 61,506,703 on the public tomato genome; or
a marker having SEQ ID NO: 20, wherein the ‘C’ at position 79 in SEQ ID NO: 20 corresponds to the physical position 61,751,657 on the public tomato genome.

6. The tomato plant of claim 1 , comprising QTL1 and QTL3 in homozygous form,
wherein the tomato plant produces a fruit comprising higher levels of anthocyanins when compared to a fruit produced by a tomato plant not carrying said QTLs in its genome, and
wherein said fruit produced by the tomato plant comprising QTL1 and QTL3 in homozygous form are not purple at the red-ripe harvest stage.

7. A propagation material that produces the plant of claim 1 ,
wherein the propagation material is suitable for sexual reproduction, and is selected from the group consisting of a microspore, a pollen, an ovary, an ovule, an embryo sac and an egg cell, or is suitable for vegetative reproduction, and is selected from the group consisting of a cutting, a root, a stem, a cell, and a protoplast,
wherein the plant produced from the propagation material comprises QTL1 and QTL3 and produces a fruit comprising higher levels of anthocyanins when compared to a fruit produced by a tomato plant not carrying said QTLs in its genome, and
wherein said fruit produced by the propagation material comprising QTL1 and QTL3 is not purple at the red-ripe harvest stage.

8. A tomato seed of the plant of claim 1 ,
wherein the seed comprises QTL1 and QTL3,
wherein the plant grown from the seed produces a fruit comprising higher levels of anthocyanins when compared to a fruit produced by a tomato plant not carrying said QTLs in its genome, and
wherein said fruit comprising QTL1 and QTL3 is not purple at the red-ripe harvest stage.

9. A tomato fruit of the plant of claim 1 , wherein said fruit is not purple at the red-ripe harvest stage.

Assignees

• Rijk Zwaan Zaadteelt En Zaadhandel Bv

Inventors

• Solleveld Johan Cornelis
• Nowosielski Agathe Anna
• Koopmans Roy

Classifications

• A01H5/08 Primary

  Fruits · CPC title

• A01H6/825 Primary

  Solanum lycopersicum [tomato] · CPC title

• C12N15/825 Primary

  involving pigment biosynthesis · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title