Cell stimulation device, cell culture device, and cell stimulation method

Patent metadata

Field	Value
Publication number	US-11485950-B2
Application number	US-201816756080-A
Country	US
Kind code	B2
Filing date	Oct 4, 2018
Priority date	Oct 25, 2017
Publication date	Nov 1, 2022
Grant date	Nov 1, 2022

Abstract

The present invention provides a cell stimulation device capable of uniformly electrically stimulating cells intended to be electrically stimulated, and of preventing medium components from decomposing and depositing.
[Solution] The cell stimulation device according to the present invention is intended to stimulate the cells being cultured in a culture medium, and is characterized in that the cell stimulation device includes an electron emission element for feeding electric charges to the culture medium via a gas phase, and an electric charge collecting electrode for collecting the electric charges from the culture medium, the electron emission element includes a lower electrode, a surface electrode, and an intermediate layer disposed between the lower electrode and the surface electrode, and the electric charge collecting electrode is disposed so as to be contactable with the culture medium.

First claim

The invention claimed is:

1. A cell stimulation device for stimulating cells being cultured in a culture medium, the cell stimulation device comprising:
an electron emission element that feeds electric charges to the culture medium via a gas phase;
an electric charge collecting electrode that collects the electric charges from the culture medium;
an insulating member; and
a needle-like terminal fixed to the insulating member, wherein:
the electron emission element comprises a lower electrode, a surface electrode, and an intermediate layer disposed between the lower electrode and the surface electrode;
the electric charge collecting electrode is disposed such that the electric charge collecting electrode is contactable with the culture medium;
the electron emission element is fixed to the insulating member; and
a tip of the needle-like terminal is in contact with the lower electrode.

2. The cell stimulation device according to claim 1 , further comprising a power supply, wherein
the power supply is disposed such that a voltage is applied between the lower electrode and the surface electrode, and a potential difference is generated between the electron emission element and the electric charge collecting electrode.

3. The cell stimulation device according to claim 2 , wherein the power supply is electrically connected to the electron emission element and the electric charge collecting electrode such that a current generated in the gas phase by feeding the electric charges from the electron emission element to the culture medium, and a current generated in the culture medium by collecting the electric charges from the culture medium by the electric charge collecting electrode form a loop current.

4. The cell stimulation device according to claim 2 , wherein the electric charge collecting electrode is grounded.

5. The cell stimulation device according to claim 1 , wherein the insulating member has a slit structure on a surface thereof.

6. The cell stimulation device according to claim 1 , wherein the electric charge collecting electrode is fixed to the insulating member.

7. A cell culture device comprising the cell stimulation device according to claim 1 , wherein:
the culture medium is contained in a culture container; and
the insulating member is disposed on the culture container such that the surface electrode is opposite to a surface of the culture medium with the gas phase interposed between the surface electrode and the surface of the culture medium.

8. The cell culture device according to claim 7 , wherein a distance between the surface electrode and the surface of the culture medium is 0.5 to 3 mm.

9. The cell culture device according to claim 7 , wherein the electric charge collecting electrode is fixed to the culture container.

10. A cell stimulation device for stimulating cells being cultured in a culture medium, the cell stimulation device comprising:
an electron emission element that feeds electric charges to the culture medium via a gas phase; and
an electric charge collecting electrode that collects the electric charges from the culture medium, wherein:
the electron emission element comprises a lower electrode, a surface electrode, and an intermediate layer disposed between the lower electrode and the surface electrode;
the electric charge collecting electrode is disposed such that the electric charge collecting electrode is contactable with the culture medium;
each of the lower electrode and the surface electrode has a plurality of elongated electrodes; and
the lower electrodes and the surface electrodes are arranged in a lattice shape such that either the lower electrodes or the surface electrodes are arranged in rows, and the other of the lower electrodes or the surface electrodes are arranged in columns.

Assignees

• Sharp Kk

Inventors

• Hirakawa Hiroyuki
• Yamamoto Haruo
• Iwamatsu Tadashi
• Sakamoto Yasuhiro
• Yamaji Hiroyuki
• Shinkawa Kohji
• Takasaki Mai
• Komaru Shohei
• Sugimori Fumiaki

Classifications

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12M1/42

  Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12M35/02 Primary

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title