Method and device comprising an optical fiber located inside a channel for determining the concentration of analyte in whole blood based on change of reflected light wavelength

Patent metadata

Field	Value
Publication number	US-11480558-B2
Application number	US-201816621009-A
Country	US
Kind code	B2
Filing date	Jun 7, 2018
Priority date	Jun 15, 2017
Publication date	Oct 25, 2022
Grant date	Oct 25, 2022

Abstract

A method and a device for determining the concentration of an analyte in whole blood is disclosed. In one embodiment, the method includes generating a plasma layer in the whole blood sample. Furthermore, the method includes exposing the plasma layer to light. The method also includes capturing light reflected from the plasma layer. Additionally, the method includes analyzing the reflected light to determine the concentration of the analyte.

First claim

What is claimed is:

1. A method of determining the concentration of an analyte in a whole blood sample, the method comprising:
flowing a whole blood sample through a channel;
generating a plasma layer in the channel, the plasma layer being devoid of blood cells;
directing light at the plasma layer;
passing the light through an optical fiber located inside the channel and extending in parallel with the channel to reflect the light off of the plasma layer multiple times;
capturing light reflected off of the plasma layer; and
analyzing the reflected light to determine the concentration of the analyte.

2. The method of claim 1 , wherein when the whole blood sample flows through the channel the blood cells migrate to the center of the channel thereby creating a plasma layer at the inner surface of the channel.

3. The method of claim 1 , wherein in generating a plasma layer in the whole blood sample, the method comprises using the optical fiber inside the channel, wherein the whole blood surrounds the optical fiber as it flows through the channel.

4. The method of claim 1 , wherein the light is directed at the plasma layer at an angle sufficient to cause a reflection off of the plasma layer.

5. The method of claim 4 , further comprising redirecting the reflected light back to the plasma layer.

6. The method of claim 1 , wherein the light is directed at the inner surface of the optical fiber.

7. The method of claim 1 , wherein in analyzing the reflected light to determine the concentration of the analyte, the method comprises:
detecting a change in the wavelength of the reflected light; and
determining the concentration of the analyte in the whole blood sample based on the wavelength of the reflected light.

8. The method of claim 1 , wherein the reflected light is captured using a spectrophotometer.

9. The method of claim 1 , wherein the analyte is hemoglobin.

10. A device for determining the concentration of an analyte in a whole blood sample, the device comprising:
a channel configured to carry whole blood;
a light source configured to direct light on the channel;
an optical fiber through which the light passes and is reflected multiple times to form reflected light, the optical fiber being located inside the channel and extending in parallel with the channel; and
a measuring unit configured to:
capture the reflected light exiting from the channel; and
compute a change in wavelength of the reflected light, wherein the change in the wavelength of the reflected light is proportional to the concentration of the analyte in the whole blood.

11. The device of claim 10 , wherein when the whole blood is flown through the channel, the blood cells migrate to the center of the channel thereby creating a plasma layer at the inner surface of the channel.

12. The device of claim 10 , wherein the channel is a fluidic channel etched on a transparent medium.

13. The device of claim 10 , wherein the channel is a tubular structure made of a transparent medium.

14. The device of claim 13 , wherein the whole blood surrounds the optical fiber as it flows through the channel.

15. The device of claim 10 , wherein the light is directed at the plasma layer at an angle sufficient to cause a reflection off of the plasma layer.

16. The device of claim 10 , wherein the measuring unit is a spectrophotometer.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Dubey Satish Kumar
• Lal Ashish Kumar
• Prabhu Vishal Manjanath
• Ledden David

Classifications

• G01N2021/052

  Tubular type; cavity type; multireflective · CPC title

• G01N2021/0346

  Capillary cells; Microcells · CPC title

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N21/552

  Attenuated total reflection · CPC title

• G01N21/05 Primary

  Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10) · CPC title