Method for inducing differentiation of corneal epithelial cells from pluripotent stem cells
US-2018010093-A1 · Jan 11, 2018 · US
Method for producing cultivated epithelial cell sheet
US11479754B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11479754-B2 |
| Application number | US-201716095173-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 19, 2017 |
| Priority date | Apr 20, 2016 |
| Publication date | Oct 25, 2022 |
| Grant date | Oct 25, 2022 |
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Abstract
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A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing an epithelial cell sheet in which cells that express an epithelial stem cell marker, a proliferating cell marker, and corneal epithelial cell marker keratin 12 and maintain a property as stem cells are present, the method comprising culturing cells derived from oral mucosal epithelium on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) a serum-free supplement comprising biotin, L-carnitine, corticosterone, ethanolamine, D(+) galactose, reduced glutathione, linoleic acid, linolenic acid, progesterone, putrescine, retinyl acetate, selenium, triiodo-L-thyronine, vitamin E, vitamin E acetate, bovine albumin, catalase, insulin, superoxide dismutase, and transferrin, (iii) a ROCK inhibitor, and (iv) one or more catechin. 2. The method according to claim 1 , wherein the medium further comprises at least one member selected from the group consisting of (v) polysaccharides, and (vi) corticoids.
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Classifications
Ophthalmic agents · CPC title
Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells (islets of Langerhans A61K35/39) · CPC title
Steroid hormones · CPC title
Kinases (EC 2.7.) · CPC title
Organic components · CPC title
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- What does patent US11479754B2 cover?
- A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.
- Who is the assignee on this patent?
- Kyoto Prefectural Public Univ Corp, Foundation For Biomedical Res And Innovation At Kobe
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0632. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 25 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).