Aminopeptidases for protein hydrolyzates

Patent metadata

Field	Value
Publication number	US-11473073-B2
Application number	US-201615574323-A
Country	US
Kind code	B2
Filing date	Jun 27, 2016
Priority date	Jun 26, 2015
Publication date	Oct 18, 2022
Grant date	Oct 18, 2022

Abstract

The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.

First claim

The invention claimed is:

1. A method for the production of a protein hydrolysate comprising subjecting a proteinaceous substrate, wherein the substrate is selected from the group consisting of casein, whey protein isolate, soy protein isolate and gluten, to a purified polypeptide having aminopeptidase activity, selected from the group consisting of:
(a) a polypeptide having an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, or SEQ ID NO: 2, or SEQ ID NO: 3, or SEQ ID NO: 4, or SEQ ID NO: 5, or SEQ ID NO: 6, or SEQ ID NO: 7, or SEQ ID NO: 8; and
(b) a fragment of (a), wherein the fragment has aminopeptidase activity.

2. The method of claim 1 , further comprising subjecting said proteinaceous substrate to an endopeptidase.

3. The method of claim 2 , wherein the hydrolysate is enriched in Leu, Gly, Glu, Ser, Asp, Asn, Pro, Cys, Ala, and/or Gln.

4. The method of claim 2 , wherein the hydrolysate is enriched in Glu and/or Gln.

5. A method for obtaining from a proteinaceous substrate a protein hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, comprising subjecting the substrate to a deamidation process and a purified polypeptide having aminopeptidase activity, selected from the group consisting of:
(a) a polypeptide having an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, or SEQ ID NO: 2, or SEQ ID NO: 3, or SEQ ID NO: 4, or SEQ ID NO: 5, or SEQ ID NO: 6, or SEQ ID NO: 7, or SEQ ID NO: 8; and
(b) a fragment of (a), wherein the fragment has aminopeptidase activity.

6. The method of claim 5 , further comprising subjecting the substrate to one or more unspecific acting endo- and/or exo-peptidase enzymes.

Assignees

• Dupont Nutrition Biosci Aps

Inventors

• Kragh Karsten Matthias
• Meinjohanns Ernst
• Eisele Thomas
• Degn Peter Edvard
• Bak Steffen Yde

Classifications

• C12C5/004

  Enzymes · CPC title

• C12N9/485 Primary

  Exopeptidases (3.4.11-3.4.19) · CPC title

• C07K2319/02

  containing a signal sequence · CPC title

• C12Y304/11

  Aminopeptidases (3.4.11) · CPC title

• C12C1/047

  Influencing the germination by chemical or physical means · CPC title