Multimeric elastin-like polypeptides

Patent metadata

Field	Value
Publication number	US-11464867-B2
Application number	US-201916274192-A
Country	US
Kind code	B2
Filing date	Feb 12, 2019
Priority date	Feb 13, 2018
Publication date	Oct 11, 2022
Grant date	Oct 11, 2022

Abstract

This disclosure provides a novel compositions and methods to deliver cyclosporine A using genetically engineered protein polymers.

First claim

What is claimed is:

1. An agent comprising a multimeric form of an elastin-like peptide (ELP) component that forms a drug binding domain stabilized by the ELP and two or more FKBP drug binding domains, wherein the agent is SEQ ID NO: 10 (5FA) or SEQ ID NO: 11 (5FV), or dimers, trimers, tetramers or pentamers thereof.

2. The agent of claim 1 , further comprising a therapeutic agent.

3. The agent of claim 2 , wherein the therapeutic agent is bound or trapped by the ELP or coacervate formed by the ELP.

4. The agent of claim 1 , further comprising a detectable label.

5. An agent comprising a polypeptide selected from SEQ ID NO: 3 (CA192), SEQ ID NO: 4 (CV96), SEQ ID NO: 8 (CAC), SEQ ID NO: 9 (3(CA)C), SEQ ID NO: 15 (4PA), or dimers, trimers, tetramers or pentamers thereof.

6. A method for delivering a therapeutic agent in vitro comprising contacting a tissue or cell with an effective amount of the agent of claim 2 .

Assignees

• Univ Southern California

Inventors

• Mackay John Andrew
• Shah Mihir
• Hamm-Alvarez Sarah F
• Guo Hao
• Peddi Santosh

Classifications

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title

• C07K16/2887

  against CD20 · CPC title

• A61K47/6801 Primary

  Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent · CPC title

• A61K31/203 Primary

  Retinoic acids {; Salts thereof} · CPC title

• C07K14/78

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title