Method for the microbial production of specific natural capsaicinoids

Patent metadata

Field	Value
Publication number	US-11459591-B2
Application number	US-201716317844-A
Country	US
Kind code	B2
Filing date	Jul 19, 2017
Priority date	Jul 19, 2016
Publication date	Oct 4, 2022
Grant date	Oct 4, 2022

Abstract

The present invention relates to the production of capsaicinoid compounds including Capsaicin and Nonivamide via microbial fermentation.

First claim

What is claimed is:

1. A method of producing a capsaicinoid glucoside of interest, said method comprising:
providing a transformed microbe culture capable of expressing 1) an ACS enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO: 1 and/or a DNA sequence similarity of at least 75% with SEQ ID NO: 3, 2) a CS enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO: 2 and/or a DNA sequence similarity of at least 90% with SEQ ID NO: 4, and 3) a CaUGT2 enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO: 6 and/or a DNA sequence similarity of at least 75% with SEQ ID NO: 5;
incubating the transformed microbe culture to induce expression of the ACS enzyme, the CS enzyme, and the CaUGT2 enzyme;
feeding a specific fatty acid precursor to said transformed microbe culture.

2. The method of claim 1 , wherein the capsaicinoid glucoside of interest is capsaicin-glucoside and the fatty acid precursor is 6E-8-methyl-6-nonenoic acid.

3. The method of claim 1 , wherein the capsaicinoid glucoside of interest is nonivamide-glucoside and the fatty acid precursor is nonanoic acid.

4. The method of claim 1 , wherein the transformed microbe culture is further capable of expressing an aminotransferase.

5. The method of claim 1 , wherein the transformed microbe culture is selected from the group consisting of yeast, non-capsaicinoid producing plants, algae and bacteria.

6. The method of claim 1 , wherein the transformed microbe culture is E. coli.

Assignees

• Conagen Inc

Inventors

• Chen Hui
• Yu Xiaodan
• Zhou Lanlan
• Wang Hongxue
• Wang Min

Classifications

• C12N9/1051

  Hexosyltransferases (2.4.1) · CPC title

• A23L27/10

  Natural spices, flavouring agents or condiments; Extracts thereof · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61K9/0014

  Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00) · CPC title

• C12N9/93

  Ligases (6) · CPC title