Microdevice for the in vivo capture of circulating cellular biomarkers

Patent metadata

Field	Value
Publication number	US-11457900-B2
Application number	US-201615547203-A
Country	US
Kind code	B2
Filing date	Feb 2, 2016
Priority date	Feb 2, 2015
Publication date	Oct 4, 2022
Grant date	Oct 4, 2022

Abstract

The invention relates to a device for capture of cellular biomarkers circulating in an animal or human biological flow, characterised in that it is suitable for use in vivo and in that it comprises a means of filtration intended to retain the said cellular biomarkers, the said means of filtration comprising at least one through-aperture the dimensions of which are suitable for retaining the said cellular biomarkers and being integral with a support, the support taking the form of a hollow component.

First claim

The invention claimed is:

1. Device for sampling of cellular biomarkers circulating in an animal or human biological flow,
wherein the cellular biomarkers are at least one of stromal and epithelial cells, circulating foetal cells (CFC), stem cells, clusters or aggregates of tumour cells, tumour cells, and monocytes (macrophages) and the device for sampling comprises at least one device for capture of cellular biomarkers attached onto a means of introduction of the at least one device for capture into a biological flow vessel,
wherein the at least one device for capture is suitable for use in vivo and in that the at least one device for capture comprises a means of filtration intended to retain the cellular biomarkers, the means of filtration comprising at least one through-aperture the dimensions of which are suitable for retaining the cellular biomarkers and the means of filtration are planar and rigidly connected to a support, the support taking the form of a hollow component open at one end and closed at another end by the means of filtration and having a rotational symmetry about an axis which is total or truncated along a plane parallel to the axis enabling the at least one device for capture to conserve laminar nature of flow through the biological flow vessel upon introduction into the biological flow vessel, and
wherein the means of filtration is configured to be inserted in a fully expanded configuration.

2. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one through-aperture has at least one transverse dimension lying between 1 μm and 100 μm.

3. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one through-aperture is substantially circular or substantially oval or substantially polygonal or takes the form of a crack.

4. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one device for capture has a rotational symmetry about an axis.

5. Device for sampling of cellular biomarkers according to claim 1 , wherein the support takes the form of a hollow cylinder, truncated along a plane parallel to the axis of the cylinder, the means of filtration closing one end of the truncated cylinder.

6. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one device for capture is made of a photosensitive resin.

7. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one device for capture is made of a biocompatible material or in that the at least one device for capture is covered with a biocompatible material.

8. Device for sampling of cellular biomarkers according to claim 1 , wherein the at least one device for capture is functionalized by means of an anti-thrombotic compound.

9. Device for sampling of cellular biomarkers according to claim 1 , wherein the means of introduction of the at least one device for capture into the blood circulation comprises a strip.

10. Device for sampling of cellular biomarkers according to claim 9 , wherein the strip comprises a metallic alloy or a composite material based on resin and fibers.

11. Device for sampling of cellular biomarkers according to claim 1 , wherein the means of introduction comprises a first means of protection of the means of filtration, intended to avoid detachment of the means of filtration.

12. Device for sampling of cellular biomarkers according to claim 11 , wherein the first means of protection comprises at least one stud attached onto the means of introduction.

13. Device for sampling of cellular biomarkers according to claim 11 , wherein the first means of protection is formed by an end piece attached to the extremity of the means of introduction.

14. Device for sampling of cellular biomarkers according to claim 13 , wherein the end piece comprises at least one arch.

15. Device for sampling of cellular biomarkers according to claim 11 , wherein the first means of protection is formed by at least one longitudinal arch which is contained by the means of introduction in the vicinity of its extremity.

16. Device for sampling of cellular biomarkers according to claim 11 , wherein the means of introduction contains a second means of protection intended to protect a blood vessel into which the device for sampling is brought to be introduced and wherein the second, or the first and the second means of protection is formed by an end piece attached to the extremity of the means of introduction.

17. Device for sampling of cellular biomarkers according to claim 11 , wherein the means of introduction contains a second means of protection intended to protect a blood vessel into which the device for sampling is brought to be introduced and wherein the second, or the first and the second means of protection is formed by at least one longitudinal arch which is contained by the means of introduction in the vicinity of its extremity.

18. Device for sampling of cellular biomarkers according to claim 1 , wherein the means of introduction contains a second means of protection intended to protect a blood vessel into which the device for sampling is brought to be introduced.

19. Device for sampling of cellular biomarkers according to claim 1 , wherein the device for sampling is covered with a biocompatible material.

20. Device for sampling of cellular biomarkers according to claim 1 , wherein the device for sampling is functionalized by means of an anti-thrombotic compound.

21. Device for sampling of cellular biomarkers according to claim 1 , wherein the device for sampling includes a means of gripping so as to render the device for sampling suitable for use in a catheter.

22. Set for sampling of cellular biomarkers comprising a catheter and a device for sampling according to claim 1 , the device for sampling being inserted into the catheter.

23. Set for sampling of cellular biomarkers according to claim 22 for use for diagnostic or therapeutic purposes.

24. Device for sampling of cellular biomarkers according to claim 1 intended to be utilized for the diagnosis or therapy of cancer.

25. Device for sampling of cellular biomarkers according to claim 1 for use for diagnostic or therapeutic purposes.

26. Device for sampling of cellular biomarkers according to claim 1 for use for diagnostic or therapeutic purposes.

27. Device for sampling of cellular biomarkers according to claim 1 , wherein the support is a cylinder, the wall of the cylinder is solid, and the cylinder is open at one end and closed at the other end by the means of filtration.

28. Device for sampling of cellular biomarkers circulating in an animal or human biological flow,
wherein the cellular biomarkers are at least one of stromal and epithelial cells, circulating foetal cells (CFC), stem cells, clusters or aggregates of tumour cells, tumour cells, and monocytes (macrophages) and the device for sampling comprises at least one device for capture of cellular biomarkers attached onto a means of introduction of the at least one device for capture into a biological flow vessel,
wherein the at least one device for capture is suitable for use in vivo and in that the at least one device for capture comprises a means of filtration intended to retain the cellular biomarkers, the means of filtration comprising at least one through-aperture the dimensions of which are suitable for retaining the cellular

Assignees

• Centre Nat Rech Scient
• Ct Hospitalier Universitaire Toulouse
• Univ Toulouse Iii—Paul Sabatier

Inventors

• Cerf Aline
• Sanson Sylvain
• Cayron Hélène
• Jimenez Alejandro Kayum
• Vieu Christophe
• Malavaud Bernard

Classifications

• A61B10/02 Primary

  Instruments for taking cell samples or for biopsy {(A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48)} · CPC title

• A61F2/01

  Filters implantable into blood vessels · CPC title

• A61F2/06

  Blood vessels · CPC title