Method of coating surfaces with nanoparticles for biological analysis of cells

Patent metadata

Field	Value
Publication number	US-11453859-B2
Application number	US-201916276167-A
Country	US
Kind code	B2
Filing date	Feb 14, 2019
Priority date	Feb 14, 2018
Publication date	Sep 27, 2022
Grant date	Sep 27, 2022

Abstract

A method of coating a surface with nanoparticles for biological analysis of cells that includes the steps of cleaning the surface with an oxidizing acid, treating the surface with an organosilane, coating the surface with nanoparticles, and then growing cells on the surface coated with the nanoparticles. The surface may be a glass surface, a silica-based surface, a plastic-based surface or a polymer-based surface. The nanoparticles may be gold-based nanomaterials.

First claim

We claim:

1. A method of coating a surface with a gold nanoparticle for biological analysis of a cell, comprising the steps of:
a. cleaning said surface with an oxidizing acid;
b. treating said cleaned surface with 3-mercaptopropyltrimethoxysilane;
C. coating said treated surface with said nanoparticle comprising a step of adding a dispersion to said surface, wherein said dispersion comprises said nanoparticle and has a pH between 8 and 10; and
d. growing said cell on said surface coated with said nanoparticle.

2. The method of claim 1 , wherein said surface is a glass surface, a silica-based surface, a plastic-based surface or a polymer-based surface.

3. The method of claim 1 , wherein said nanoparticle comprises a nanocage.

4. The method of claim 1 , further comprising the step of conducting a biological analysis of said cell.

5. The method of claim 4 , wherein said biological analysis comprises exposing said cell to radiation.

6. The method of claim 1 , wherein said pH is 9.

Assignees

• Bioventures Llc

Inventors

• Jenkins Samir V
• Griffin Robert J
• Borrelli Michael J

Classifications

• C12N5/0619

  Neurons · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• C12Q1/025

  for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics (antimicrobial activity C12Q1/18) · CPC title

• C12N5/0075 Primary

  using microcarriers · CPC title

• G01N33/5005 Primary

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title