Method for improving transformation efficiency of plant and method for transforming plant

Patent metadata

Field	Value
Publication number	US-11447784-B2
Application number	US-201816617930-A
Country	US
Kind code	B2
Filing date	Jun 7, 2018
Priority date	Jun 7, 2017
Publication date	Sep 20, 2022
Grant date	Sep 20, 2022

Abstract

The present invention provides a method for improving transformation efficiency of a plant. The method according to the present invention comprises the use of a nucleic acid encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4, or a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 2 or 4, and having a function of improving transformation efficiency of a plant.

First claim

The invention claimed is:

1. A method for improving transformation efficiency of a plant, comprising:
(a) introducing into a plant
(1) a nucleic acid construct that comprises a nucleic acid encoding a polypeptide comprising the amino acid sequence according to SEQ ID NO: 2, and a promoter for producing said first nucleic acid in a plant, wherein said promoter is heterologous with respect to said nucleic acid; and
(2) a desired nucleic acid to be expressed in the plant; and
(b) selecting from a plurality of cells of said plant a transformed plant cell with an improved transformation efficiency, wherein the improvement comprises at least one improvement selected from the group consisting of:
a) improvement of efficiency of callus formation of the plant;
b) improvement of redifferentiation rate of the plant; and
c) improvement of gene transfer efficiency.

2. The method for transformation according to claim 1 , wherein the desired nucleic acid to be produced in the plant is transiently expressed.

Assignees

• Inst Crop Science Caas
• Japan Tobacco Inc
• Kaneka Corp

Inventors

• Ye Xingguo
• Wang Ke
• Ishida Yuji
• Yanagihara Chizu
• Liu Huiyun
• Wang Kunyang
• Shi Lei
• Du Lipu
• Wang Jing
• Lin Zhishan

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8201 Primary

  Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation · CPC title

• C12N15/8216

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• A01H4/005

  Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques · CPC title

• C12N15/8207

  by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers · CPC title