Transparent disc-shaped microparticles coated with polydopamine

Patent metadata

Field	Value
Publication number	US-11447733-B2
Application number	US-201816486600-A
Country	US
Kind code	B2
Filing date	Jan 15, 2018
Priority date	Feb 17, 2017
Publication date	Sep 20, 2022
Grant date	Sep 20, 2022

Abstract

The present invention relates to transparent disc-shaped microparticles having polydopamine-modified surfaces and cell culture using the same. When the disc-shaped microparticles coated with polydopamine according to the present invention are used in cell culture, cell adhesive properties are improved and an inverted microscope may be employed to observe cells, which are major advantages when compared to conventional spherical microcarriers.

First claim

The invention claimed is:

1. A disc-shaped microparticle for cell culture, comprising a polydopamine-coated surface layer, wherein the disc-shaped microparticle is formed by photocuring polymerization of a photocurable substance having an acrylate functional group and a linker having a functional group polymerizable with the photocurable substance and an alkoxysilyl group,
wherein the disc-shaped microparticle has a thickness of 5 μm to 300 μm and a diameter of 200 μm to 3,000 μm,
wherein the polydopamine-coated surface layer is formed for a time period of 40 to 80 minutes,
wherein the disc-shaped microparticle on which the polydopamine-coated surface layer is formed is configured to be cultured in three dimensions, and a density and shape of cells attached thereto can be observed by an inverted microscope, and
wherein the disc-shaped microparticle for cell culture is optically transparent.

2. The disc-shaped microparticle according to claim 1 , wherein the disc-shaped microparticle comprises a coded polymeric microparticle core and a silica shell, wherein a carboxyl group or an amine group is introduced to a surface of the silica shell.

3. A method of preparing disc-shaped microparticles for cell culture having polydopamine-coated surfaces, the method comprising:
(a) a step of preparing microparticles by photocuring polymerization of a mixture of a photocurable substance and a linker having a functional group polymerizable with the photocurable substance and an alkoxysilyl group; and
(b) a step of adding a dopamine hydrochloride solution to the microparticles and stirring,
wherein the stirring of step (b) is performed for a time period of 40 to 80 minutes,
wherein the disc-shaped microparticle has a thickness of 5 μm to 300 μm and a diameter of 200 μm to 3,000 μm,
wherein the disc-shaped microparticle on which the polydopamine-coated surface layer is formed is configured to be cultured in three dimensions, and a density and shape of cells attached thereto can be observed by an inverted microscope, and
wherein the disc-shaped microparticle for cell culture is optically transparent.

4. The method according to claim 3 , wherein the disc-shaped microparticle comprises a coded polymeric microparticle core and a silica shell, wherein a carboxyl group or an amine group is introduced to a surface of the silica shell.

Assignees

• Univ Industry Cooperation Group Kyung Hee Univ

Inventors

• Oh Tong In
• Kim Sung Hyun
• Park Wook
• Lee Eun Ah

Classifications

• C12N11/089

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12N5/0062

  General methods for three-dimensional culture · CPC title

• C12M25/16 Primary

  Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00) · CPC title

• C12N2537/10

  Cross-linking · CPC title