Biocompatible sculptured extracellular nanomatrix enables self assembly of neural stem cells into miniature brain organoids of substantia nigra

Patent metadata

Field	Value
Publication number	US-11434470-B2
Application number	US-201816209934-A
Country	US
Kind code	B2
Filing date	Dec 4, 2018
Priority date	May 23, 2016
Publication date	Sep 6, 2022
Grant date	Sep 6, 2022

Abstract

Biocompatible silica inorganic sculptured extracellular nanomatrices (iSECnMs) of silica nanozigzags are deposited by glancing angle deposition (GLAD), to achieve induction of specific differentiation without growth factors. The nanostructure includes a plurality of nanozigzags. The nanozigzags include SiO 2 and the nanozigzags having a pitch of 80 nm to 250 nm, and a contact depth of 90 nm to 260 nm. A method of cell therapy including substantia nigra organoids formed on silica iSECnMs is also provided.

First claim

The invention claimed is:

1. A nanostructure comprising a plurality of nanozigzags, wherein the nanozigzags comprise SiO 2 having a stiffness of 19.7±2.3 μN/nm and the nanozigzags have a pitch of 80 nm to 250 nm, and a contact depth of 90 nm to 260 nm.

2. The nanostructure according to claim 1 , wherein the nanozigzags comprise at least three pitches.

3. The nanostructure according to claim 1 , wherein the nanozigzags having a pitch of 80 nm to 225 nm.

4. The nanostructure according to claim 3 , wherein the nanozigzags having a pitch of 80 nm, 170 nm or 225 nm.

Assignees

• Univ Hong Kong Baptist Univ

Inventors

• Yung Kin Lam
• Huang Zhifeng
• Zhang Shi Qing

Classifications

• C12N2535/00

  Supports or coatings for cell culture characterised by topography · CPC title

• C12N5/0619 Primary

  Neurons · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C12N5/0697

  Artificial constructs associating cells of different lineages, e.g. tissue equivalents (blood vessels C12N5/0691) · CPC title

• C12N2533/10

  Mineral substrates · CPC title