Bubble eliminating structure, bubble eliminating method, and agitating method using the same

Patent metadata

Field	Value
Publication number	US-11426721-B2
Application number	US-201716313485-A
Country	US
Kind code	B2
Filing date	Jun 26, 2017
Priority date	Jul 25, 2016
Publication date	Aug 30, 2022
Grant date	Aug 30, 2022

Abstract

Provided are a bubble eliminating structure and a bubble eliminating method which eliminate bubbles in a liquid by agitating the liquid, and an agitating method using the same. A first groove 114, which is an upstream bubble eliminating groove, and a second groove 131, which is a downstream bubble eliminating groove, are branched from a mixing well 13. After starting suction of the liquid from mixing well 13 into the first groove 114, suction of the liquid from the mixing well 13 into the second groove 131 is started, and after completion of discharge of the liquid from the first groove 114 into the mixing well 13, discharge of the liquid from the second groove 131 into the mixing well 13 is completed. This operation is repeated to eliminate bubbles.

First claim

The invention claimed is:

1. A bubble eliminating method using a bubble eliminating structure, the bubble eliminating structure including an analysis chip installed on an upper surface side of an elastic membrane, and a driving portion installed on a lower surface side of the elastic membrane, the analysis chip including a vessel provided on an upper surface of the analysis chip, and a first groove and a second groove that are provided on a lower surface of the analysis chip and are branched from the vessel, the bubble eliminating method comprising:
holding a liquid in the vessel;
starting suction of the liquid from the vessel into the first groove;
starting suction of the liquid from the vessel into the second groove after the starting of the suction of the liquid from the vessel into the first groove, while a portion of the liquid remains in the vessel;
discharging the liquid from the first groove into the vessel; and
discharging the liquid from the second groove into the vessel after completion of the discharging of the liquid from the first groove into the vessel.

2. The bubble eliminating method according to claim 1 ,
wherein the driving portion includes a plurality of recesses provided on the upper surface of the driving portion, the plurality of recesses including a first recess and a second recess that are coupled to the first groove and the second groove, respectively, through the elastic membrane, a third recess coupled to the first recess, and a fourth recess coupled to the third recess,
the method further comprising:
decompressing the third recess while the first recess is pressurized;

after the third recess is decompressed while the first recess is pressurized, pressurizing the third recess while the pressurizing the fourth recess.

3. The bubble eliminating method according to claim 1 , further comprising:
repeatedly suctioning the liquid into the first groove and the second groove and discharging the liquid from the first groove and the second groove.

4. The bubble eliminating method according to claim 1 , wherein
the driving portion includes a plurality of recesses provided on the upper surface of the driving portion, the plurality of recesses including a first recess and a second recess that are coupled to the first groove and the second groove, respectively, through the elastic membrane,
the method further comprising:
after the first recess is decompressed to start suction of a liquid from the vessel into the first groove, the second recess is decompressed to start suction of a liquid from the vessel into the second groove, and
after the first recess is pressurized to complete suction of a liquid from the first groove into the vessel, the second recess is pressurized to complete discharge of a liquid from the second groove into the vessel.

5. The bubble eliminating method according to claim 4 , further comprising:
controlling an open state and a closed state of each of a pressurizing solenoid valve and a decompressing solenoid valve that correspond to the first recess and the second recess, respectively to perform decompression and pressurization of the first recess and the second recess.

6. The bubble eliminating method according to claim 5 , further comprising:
opening the decompressing solenoid valve corresponding to the first recess to the open state to decompress the first recess and generating a gap between the elastic membrane and the analysis chip,
after completion of suction of the liquid from the vessel into the first recess, opening the decompressing solenoid valve corresponding to the second recess to the open state to decompress the second recess and generating a gap between the elastic membrane and the analysis chip, and
after completion of suction of the liquid from the vessel into the second recess, the decompressing solenoid valve corresponding to the first recess is brought into the closed state.

7. The bubble eliminating method according to claim 6 , further comprising:
after the decompressing solenoid valve corresponding to the first recess is brought into the closed state, opening the pressurizing solenoid valve corresponding to the first recess to the open state thereby pressurizing the first recess.

8. The bubble eliminating method according to claim 5 , further comprising:
opening the decompressing solenoid valve corresponding to the first recess is to the open state to decompress the first recess and generating a gap between the elastic membrane and the analysis chip,
after suction of the liquid from the vessel into the first recess is started, opening the decompressing solenoid valve corresponding to the second recess to the open state to start decompression of the second recess and generating a gap between the elastic membrane and the analysis chip, and
before completion of suction of the liquid from the vessel into the second recess, closing the decompressing solenoid valve corresponding to the first recess to the closed state.

Assignees

• Hitachi High Tech Corp

Inventors

• Nagaoka Yoshihiro
• Nakazawa Taro
• Miyazaki Mitsuhiro
• Kimura Ryusuke
• Yamazaki Motohiro

Classifications

• G01N35/08

  using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis · CPC title

• B01L2400/0666

  Solenoid valves · CPC title

• B01L3/567

  Valves, taps or stop-cocks · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title