Centripetal microfluidic platform for LAL reactive substances testing

Patent metadata

Field	Value
Publication number	US-11422133-B2
Application number	US-201916660405-A
Country	US
Kind code	B2
Filing date	Oct 22, 2019
Priority date	Oct 8, 2012
Publication date	Aug 23, 2022
Grant date	Aug 23, 2022

Abstract

A centripetal microfluidic platform comprised of a microfluidics disc and a reader for testing LAL-reactive substances in fluid samples is provided. The microfluidic disc may comprise at least two testing areas wherein each testing area includes a reservoir portion for receiving at least one fluid sample. The disc may comprise a distribution network portion in fluid communication with the reservoir portion. Each distribution network portion may comprise a distribution network of at least four (4) channels, wherein each channel has a metering portion and at least one analysis chamber portion. The analysis chamber portion may comprise a mixing chamber for mixing samples and reagents and an optical chamber portion that is compatible with an optical reader. The metering portion may be sized to meter an aliquot of the fluid sample for analysis in the analysis chamber portion. At least one analysis chamber portion has at least one reagent isolated therein. The centripetal microfluidic platform further includes a reader for testing fluid samples within a microfluidic disc comprising an enclosure, an optical bench, a centripetal disc drive, and a controller. A method for testing at least one fluid sample for LAL-reactive substances is also provided.

First claim

What is claimed is:

1. A method for testing at least one fluid sample for a concentration of Limulus amebocyte lysate (LAL)-reactive substances, said method comprising:
inserting a microfluidic disc into a optical reader wherein:
said microfluidic disc comprises at least two testing areas wherein each testing area includes:
a reservoir portion for receiving at least one fluid sample, said reservoir portion comprising a reservoir and a reservoir outlet;
and a distribution network portion in fluid communication with said reservoir portion;
wherein each distribution network portion comprises a distribution network of at least four (4) testing channels, wherein each channel has a metering portion and at least one analysis chamber portion comprising an optical chamber, said metering portion being sized to meter an aliquot of said fluid sample for analysis in said optical chamber;
wherein said distribution network portion further comprises a main distribution channel in fluid communication with said reservoir outlet, said metering portions are in fluid communication with said main distribution channel, a waste inlet channel in fluid communication with said main distribution channel, and a waste chamber in fluid communication with said waste inlet channel for confining any excess of said fluid sample and separating said excess fluid sample from said aliquot; and
said reader comprises an enclosure, an optical bench, a centripetal disc drive, an inlet for introducing said fluid sample into said disc, and a controller;

inserting said fluid sample into said inlet of said reader;
spinning said disc until reaction velocity is reached;
analyzing said aliquot in said optical chamber using said optical bench to obtain measurement data and/or reaction data;
calculating said concentration of LAL-reactive substances using calibration curves and said measurement data and/or reaction data; and
reporting and/or storing said concentration of LAL-reactive substances.

2. The method of claim 1 , further comprising:
inserting at least one reagent comprising a detection reagent and/or LAL-reactive substance into said inlet of said reader;
spinning said disc until reaction velocity is reached;
allowing said aliquot to react with said detection reagent;
analyzing said aliquot in said optical chamber using said optical bench to obtain measurement data and/or reaction data;
calculating said concentration of LAL-reactive substances using calibration curves and said measurement data and/or reaction data; and
reporting and/or storing said concentration of LAL-reactive substances.

3. The method of claim 1 , wherein at least one testing channel has at least one reagent isolated therein, said reagent comprising a LAL-reactive substance and/or a detection reagent and wherein said method further comprises:
transferring said fluid sample from said reservoir to said metering portion and metering said aliquot;
transferring said aliquot from said metering portion to said optical chamber;
continuously monitoring said aliquot in said optical chamber using said optical bench until said aliquot has finished reacting to obtain measurement data and/or reaction data;
calculating said concentration of LAL-reactive substances using calibration curves and said measurement data and/or reaction data; and
reporting and/or storing said concentration of LAL-reactive substances.

4. The method of claim 1 , wherein said measurement data and/or reaction data comprises, aliquot volumes, reaction kinetics, fluid motions, transmission, absorption, optical density, color, color value, hue, spectrum, turbidity, scattered light, chemiluminescence, fluorescence, and magnetic resonance.

5. The method of claim 4 , wherein said method and/or said measurement data and/or reaction data are validated using historical measurement data and/or data from known reaction kinetics.

6. The method of claim 5 , wherein a tracer is immobilized within each of said testing channels to aid in measuring and validating a volume of said aliquots.

Assignees

• Bl Technologies Inc

Inventors

• Melanson Paul Charles
• Godec Richard Douglas
• Stonesmith Matthew Kaddeland
• Smith Darren Barry
• Sun Chao

Classifications

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01L2400/0406

  capillary forces · CPC title

• B01L2400/0694

  vents used to stop and induce flow, backpressure valves · CPC title

• B01L2200/0684

  Venting, avoiding backpressure, avoid gas bubbles · CPC title

• B01L2300/021

  Identification, e.g. bar codes · CPC title