High-purity mogrosides and process for their purification

Patent metadata

Field	Value
Publication number	US-11419351-B2
Application number	US-201514792594-A
Country	US
Kind code	B2
Filing date	Jul 6, 2015
Priority date	Sep 3, 2010
Publication date	Aug 23, 2022
Grant date	Aug 23, 2022

Abstract

The present invention provides a process for preparation of highly purified mogrosides mixture from low purity mogrosides mixture. The process comprises providing a mixture of low purity mogrosides, dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to form an initial solution of mogrosides, passing the initial solution through a column system, wherein the column system comprises a plurality of columns, and each column is packed with a sorbent having different affinities to impurities and mogrosides so that one or more columns retains more mogrosides than other columns, washing the columns to remove impurities with an acidic aqueous solution, a basic aqueous solution, and an aqueous alcoholic solution successively, eluting the columns with an aqueous alcohol solution that contains higher alcohol content than the aqueous alcohol solution used in the washing step, wherein the eluate from the columns with high content of mogrosides are combined, and drying the combined eluate to obtain high purity mogrosides with the content of the total mogrosides are more than 70% (w/w). The present invention also provides a sweetener mixture and product comprising high purity mogrosides.

First claim

I claim:

1. A sweetener composition comprising high purity mogrosides having a mogroside V content of at least 60% (w/w) on a dry basis; a 11-oxo-Mogroside V content which is higher than in low purity mogrosides; and a total mogroside content of more than 70% (w/w) on a dry basis; wherein the sweetener composition is prepared by a process comprising the steps of:
a. providing a mixture of low purity mogrosides having an initial mogroside V and 11-oxo-Mogroside V to total mogroside ratio;
b. dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to an initial solution of mogrosides;
c. passing the initial solution through a column system, wherein the column system comprises a plurality of columns, wherein the plurality of columns are sequentially coupled, thus when the initial solution passes through the column system, it passes through the plurality of columns in a continuous and sequential manner; wherein the plurality of columns are packed with a macroporous polymeric adsorption resin capable of adsorbing mogrosides; and wherein, when the initial solution passes through the plurality of columns, each column of the plurality of columns retains a different ratio of mogrosides from the other columns;
d. physically separating the plurality of columns into individual columns;
e. separately eluting the individual columns with an aqueous alcohol solution, wherein the retention of mogrosides in each of the plurality of columns is determined so that the eluates from the one or more columns having a mogroside V and 11-oxo-Mogroside V to total mogroside ratio of greater than the initial mogroside V and 11-oxo-Mogroside V to total mogroside ratio are combined; and
f. drying the combined eluates to obtain high purity mogrosides.

2. The sweetener composition of claim 1 , further comprising a high intensity sweetener, wherein the high intensity sweetener is selected from the group consisting of steviol glycosides including a purified sweet steviol glycoside mixture, stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, dulcoside A, dulcoside B, rubusoside, and stevia; siamenoside; mogroside IV; mogroside V; Luo Han Guo sweetener; monatin and its salts (monatin SS, RR, RS, SR); glycyrrhizic acid and its salts; curculin; thaumatin; monellin; mabinlin; brazzein; hernandulcin; phyllodulcin; glycyphyllin; phloridzin; trilobatin; baiyunoside; osladin; polypodoside A; pterocaryoside A; pterocaryoside B; mukurozioside; phlomisoside I; periandrin I; abrusoside A; cyclocarioside I; and combinations thereof.

3. The sweetener composition of claim 1 , wherein the process further comprises a step of combining the sweetener composition with a consumable product.

4. The sweetener composition of claim 3 , wherein the consumable product is selected from the group consisting of food, beverage, pharmaceutical composition, tobacco, nutraceutical, oral hygienic composition, or cosmetic.

Assignees

• Purecircle Sdn Bhd

Inventors

• Markosyan Avetik

Classifications

• A23V2250/24

  Non-sugar sweeteners · CPC title

• A61K8/63

  Steroids; Derivatives thereof · CPC title

• A23L2/60 Primary

  Sweeteners · CPC title

• A61K8/9789

  Magnoliopsida [dicotyledons] · CPC title

• A61K8/9794

  Liliopsida [monocotyledons] · CPC title