Porous membrane enabled mass spectrometry characterization of microfluidic devices

Patent metadata

Field	Value
Publication number	US-11417508-B2
Application number	US-202016744980-A
Country	US
Kind code	B2
Filing date	Jan 16, 2020
Priority date	Jan 16, 2020
Publication date	Aug 16, 2022
Grant date	Aug 16, 2022

Abstract

A system for sampling a liquid includes a sample fluid conduit including a membrane having pores. The membrane prevents the passage of the sample liquid through the pores at a first pressure of the sample liquid in the sample fluid conduit. A surface sampling capture probe has a distal end. The capture probe includes a solvent supply conduit and a solvent exhaust conduit. A solvent composition flowing at the distal end of the capture probe establishes a liquid junction with the membrane and establishes a second pressure within the liquid junction at the membrane. The second pressure is lower than the first pressure. Sample liquid will be drawn through the pores of the membrane by the second pressure at the liquid junction. A method for sampling a liquid and for performing chemical analysis on a liquid are also disclosed.

First claim

We claim:

1. A method for sampling a sample liquid, comprising the steps of:
providing the sample liquid through a sample fluid conduit having an internal sample fluid flow channel, the sample fluid conduit comprising a membrane comprising pores, the membrane preventing the passage of the sample liquid through the pores at a first pressure of the sample liquid in the sample fluid flow channel of the sample fluid conduit;
providing a surface sampling capture probe with a distal end, the capture probe comprising a solvent supply conduit with an open end and a solvent exhaust conduit with an open end;
flowing solvent composition at the distal end of the capture probe from the open end of the solvent supply conduit to the open end of the exhaust conduit and establishing a liquid junction with the membrane and establishing a second pressure within the liquid junction at the membrane, the second pressure being lower than the first pressure;
drawing sample liquid from the sample fluid flow channel through the pores of the membrane by the second pressure at the liquid junction wherein the extracted sample liquid is combined with the flowing solvent composition of the capture probe and flows into the open end of the exhaust conduit of the capture probe.

2. The method of claim 1 , further comprising the step of conducting mass spectrometry on the extracted sample liquid.

3. The method of claim 1 , wherein the surface tension of the solvent composition when in a liquid junction with the membrane having a junction fluid diameter prevents expansion of the junction fluid diameter beyond two times the diameter of the distal end of the capture probe.

4. The method of claim 1 , wherein the surface tension of the solvent composition does not permit the solvent to flow through the pores of the membrane at the second pressure.

5. The method of claim 1 , further comprising the step of collecting the solvent composition and sample liquid from the exhaust conduit of the capture probe in a storage container.

6. The method of claim 1 , wherein the solvent composition comprises a component that is a solvent for an analyte of interest in the sample liquid.

7. The method of claim 1 , wherein the solvent composition comprises a solvent liquid and a high surface tension component having a surface tension higher than that of the solvent liquid.

8. The method of claim 1 , wherein the solvent composition comprises an acid or base component for ionizing an analyte of interest in the sample fluid.

9. The method of claim 1 , wherein the solvent composition comprises a reactant for an analyte of interest in the sample liquid to produce an analyte reaction product which analyte reaction product provides increased sensitivity for mass spectrometry relative to the unreacted analyte of interest.

10. A system for sampling a liquid, comprising:
a sample fluid conduit having an internal sample fluid flow channel, the sample fluid conduit comprising a membrane comprising pores, the membrane preventing the passage of the sample liquid through the pores at a first pressure of the flowing sample liquid in the sample fluid flow channel of the sample fluid conduit;
a surface sampling probe with a distal end, the capture probe comprising a solvent supply conduit with an open end and a solvent exhaust conduit with an open end, wherein a solvent composition flowing at the distal end of the capture probe from the open end of the solvent supply conduit to the open end of the exhaust conduit establishes a liquid junction with the membrane and establishes a second pressure within the liquid junction at the membrane, the second pressure being lower than the first pressure;
wherein sample liquid will be drawn through the pores of the membrane by the second pressure at the liquid junction and the extracted sample liquid will combine with the flowing solvent composition of the capture probe and flow into the open end of the exhaust conduit of the capture probe.

11. The system of claim 10 , further comprising a chemical analysis device.

12. The system of claim 11 , wherein the chemical analysis device comprises a mass spectrometer.

13. The system of claim 10 , wherein the surface tension of the solvent composition when in a liquid junction with the membrane having a junction fluid diameter prevents expansion of the junction fluid diameter beyond two times the diameter of the distal end of the capture probe.

14. The system of claim 10 , wherein the solvent supply conduit of the surface sampling probe comprises an outer probe housing, and the solvent exhaust conduit comprises a coaxial inner solvent exhaust conduit, and an annular solvent supply flow passage between at least a portion of the solvent exhaust conduit and at least a portion of the outer probe housing.

15. The system of claim 10 , wherein the surface tension of the solvent composition does not permit the solvent composition to flow through the pores of the membrane at the second pressure.

16. The system of claim 10 , further comprising a storage container for collecting the solvent composition and sample liquid from the exhaust conduit of the capture probe.

17. The system of claim 10 , wherein the solvent composition comprises a component that is a solvent for an analyte of interest in the sample liquid.

18. The system of claim 10 , wherein the solvent composition comprises a high surface tension component.

19. The system of claim 10 , wherein the solvent composition comprises an acid or base component for ionizing an analyte of interest in the sample fluid.

20. The system of claim 10 , wherein the solvent composition comprises a reactant for an analyte of interest in the sample liquid to produce an analyte reaction product which analyte reaction product provides increased sensitivity for mass spectrometry relative to the unreacted analyte of interest.

21. A method for sampling a sample liquid, comprising the steps of:
flowing the sample liquid through a sample fluid flow channel of a sample fluid conduit, the sample fluid conduit comprising a membrane comprising pores, the membrane preventing the passage of the sample liquid through the pores at a first pressure of the sample liquid in the sample fluid flow channel of the sample fluid conduit;
providing a surface sampling capture probe with a distal end, the capture probe comprising a solvent supply conduit with an open end and a solvent exhaust conduit with an open end;
flowing a solvent composition at the distal end of the capture probe from the open end of the solvent supply conduit to the open end of the exhaust conduit and establishing a liquid junction with the membrane and establishing a second pressure within the liquid junction at the membrane, the second pressure being lower than the first pressure;
wherein sample liquid is drawn through the pores of the membrane by the second pressure at the liquid junction and wherein the extracted sample liquid is combined with the flowing solvent composition of the capture probe and flows into the open end of the exhaust conduit of the capture probe;
directing the extracted sample liquid to a chemical analysis device and performing chemical analysis on the extracted sample liquid.

22. The method of claim 21 , wherein the chemical analysis device is a mass spectrometer and the chemical analysis is mass spectrometry.

23. The method of claim 21 , wherein the surface tension of the solvent composition when in a liquid junction with the membrane having a junction

Assignees

• Ut Battelle Llc

Inventors

• Cahill John F
• Kertesz Vilmos
• Retterer Scott T

Classifications

• G01N1/10

  in the liquid or fluent state {(burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00)} · CPC title

• G01N35/1095

  for supplying the samples to flow-through analysers (for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00) · CPC title

• G01N2030/009

  Extraction · CPC title

• H01J49/0454 Primary

  with means for vaporising using mechanical energy, e.g. by ultrasonic vibrations · CPC title

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title