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Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger
US11414653B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11414653-B2 |
| Application number | US-201816124127-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 6, 2018 |
| Priority date | Sep 6, 2017 |
| Publication date | Aug 16, 2022 |
| Grant date | Aug 16, 2022 |
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Abstract
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The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
First claim
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What is claimed is: 1. An Aspergillus niger host cell comprising a nucleic acid encoding a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to the Aspergillus niger host cell. 2. The Aspergillus niger cell of claim 1 , wherein the ascomycete fungi is an Aspergillus species. 3. The Aspergillus niger cell of claim 2 , wherein the Aspergillus species is an Aspergillus niger. 4. The Aspergillus niger host cell of claim 3 , wherein the Aspergillus niger is an Aspergillus niger strain 1015. 5. The Aspergillus niger host cell of claim 1 , wherein the ecm33 promoter comprises the nucleotide sequence of SEQ ID NO:1. 6. The Aspergillus niger host cell of claim 1 , wherein the gene of interest is a glycoside hydrolase enzyme. 7. The Aspergillus niger host cell of claim 6 , wherein the glycoside hydrolase enzyme is a glucosidase. 8. The Aspergillus niger host cell of claim 1 , wherein the ecm33 promoter is heterologous to Aspergillus niger host cell. 9. The Aspergillus niger host cell of claim 8 , wherein the gene of interest is heterologous to Aspergillus niger host cell. 10. The Aspergillus niger host cell of claim 1 , wherein the gene of interest is heterologous to Aspergillus niger host cell. 11. A method of expressing a heterologous gene of interest in an Aspergillus niger host cell, comprising: (a) introducing a nucleic acid encoding a queen of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the queen of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger , into an A. niger host cell, and (b) culturing or growing the host cell in a medium for expressing the gene of interest. 12. The method of claim 11 , further comprising (c) separating or purifying a gene product encoded by the gene of interest from the host cell and/or medium.
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- What does patent US11414653B2 cover?
- The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
- Who is the assignee on this patent?
- Univ California, Nat Tech & Eng Solutions Sandia Llc, Pacific Northwest Nat Laboratory Pnnl, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/80. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 16 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).