Microorganism preparation feeding method, microorganism preparation automatic feeding apparatus, and wastewater processing system

Patent metadata

Field	Value
Publication number	US-11414332-B2
Application number	US-201816612172-A
Country	US
Kind code	B2
Filing date	May 9, 2018
Priority date	May 9, 2017
Publication date	Aug 16, 2022
Grant date	Aug 16, 2022

Abstract

The microorganism preparation feeding method of the invention employs an automatic microorganism preparation feeding apparatus which includes a cold storage apparatus for refrigeration-storing a seed microorganism belonging to the aerobic microorganism group including at least one species of aerobic microorganisms capable of decomposing oil and fat contained in oil/fat-including wastewater and a growth tank for growing the seed microorganism so as to produce the microorganism preparation, wherein the seed microorganism belonging to the aerobic microorganism group is maintained in a live state by means of the cold storage apparatus, the seed microorganism is periodically grown by means of the growth tank so as to produce a predetermined microorganism preparation, and the produced predetermined microorganism preparation is fed to the oil/fat-including wastewater. The method includes refrigeration-storing, as the seed microorganism, a microorganism whose population density is 1×107 CFU/mL to 5×109 CFU/mL in the cold storage apparatus; growing, as a source material, the seed microorganism of a predetermined volume by means of the growth tank so as to produce the predetermined microorganism preparation whose volume is 50 to 500 times the predetermined volume of the seed microorganism and whose population density is 1×107 CFU/mL to 2×1010 CFU/mL; and feeding the produced microorganism preparation to the oil/fat-including wastewater.

First claim

The invention claimed is:

1. A microorganism preparation feeding method characterized by use of a growth tank for growing a seed microorganism belonging to an aerobic microorganism group including at least one species of aerobic microorganisms capable of decomposing oil and fat contained in oil/fat-including wastewater, wherein the seed microorganism belonging to the aerobic microorganism group is maintained in a live state, the seed microorganism is grown in the growth tank by steps of cleaning the growth tank, storing water in the growth tank, and feeding to the growth tank the seed microorganism, so as to produce a microorganism preparation, and the produced microorganism preparation is fed to the oil/fat-including wastewater, the method comprising: feeding to the growth tank, as a source material, the seed microorganism which is maintained in the live state by means of a cold storage apparatus being controlled so that the temperature is 0° C. to 20° C. and whose population density is 1×10 7 CFU/mL to 5×10 9 CFU/mL in the cold storage apparatus; growing the seed microorganism in a seed volume by means of the growth tank so as to produce the microorganism preparation whose volume is 50 to 500 times the seed volume of the seed microorganism and whose population density is 1×10 7 CFU/mL to 2×10 10 CFU/mL; and feeding the produced microorganism preparation to the oil/fat-including wastewater; wherein the seed microorganism is maintained in the live state by introducing air into the seed microorganism of the cold storage apparatus and generating convection for agitating and mixing the seed microorganism.

2. The microorganism preparation feeding method according to claim 1 , wherein the growth of the seed microorganism in the growth tank is performed by use of the seed microorganism in the seed volume, water, an activating agent for activating the seed microorganism, and a carbon source composed of vegetable oil and growing the seed microorganism; and introducing air into the seed microorganism and generating convection for agitating and mixing the seed microorganism.

3. The microorganism preparation feeding method according to claim 2 , wherein the activating agent contains nitrogen, phosphorus, and potassium.

4. The microorganism preparation feeding method according to claim 1 , wherein the growth of the seed microorganism is repeatedly performed every day such that the seed microorganism is grown once every 24 hours.

5. The microorganism preparation feeding method according to claim 1 , wherein the method comprises: a cleaning step of cleaning the growth tank at a first timing; a water storage step of storing water of a predetermined amount in the growth tank at a second timing; a growing step of supplying at a third timing to the growth tank the seed microorganism in the seed volume, an activating agent for activating the seed microorganism, and a carbon source composed of vegetable oil and growing the seed microorganism in respective predetermined amounts, and growing the seed microorganism to have a population density in a predetermined range by introducing air into a resultant mixture simultaneously with agitating and mixing the resultant mixture in a state in which the resultant mixture is maintained at a constant temperature; and a feeding step of feeding the microorganism preparation produced in the growing step to the oil/fat-including wastewater in a predetermined amount at a fourth timing.

6. The microorganism preparation feeding method according to claim 5 , wherein the cleaning step, the water storage step, the growing step, and the feeding step are repeatedly performed every day such that each of the steps is performed once every 24 hours.

7. The microorganism preparation feeding method according to claim 5 , wherein each of the cleaning step, the water storage step, the growing step, and the feeding step is repeatedly performed at intervals of 48 hours in each of two growth tanks such that each step is performed once every 48 hours and a time shift of 24 hours is present between each of the steps performed in one growth tank and a corresponding one of the steps performed in the other growth tank, whereby the microorganism preparation is fed to the oil/fat-including wastewater from the two growth tanks every day.

8. The microorganism preparation feeding method according to claim 5 , further comprising a dechlorination step of performing aeration for a predetermined period of time after tap water has been supplied to and stored in the growth tank, thereby removing chlorine from the tap water.

9. The microorganism preparation feeding method according to claim 1 , wherein the growth tank is a tubular growth tank whose entirety is elongated in the vertical direction, and comprises a diffuser which has a plurality of air discharge openings provided at an end of an air discharge portion located at a lower region of the growth tank so as to introduce air into contents of the growth tank and generate convection for agitating and mixing the microorganism preparation.

10. The microorganism preparation feeding method according to claim 1 , wherein the aerobic microorganism group includes Yarrowia lipolytica 1A1 strain NITE BP-1167.

11. The microorganism preparation feeding method according to claim 1 , wherein the aerobic microorganism group includes Burkholderia arboris SL1B1 strain NITE BP-00724.

Assignees

• Nissan Chemical Corp

Inventors

• FUJIOKA Masatake

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12N1/16

  Yeasts; Culture media therefor · CPC title

• C12M39/00

  Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms (apparatus for cleaning laboratory receptacles or instruments B01L13/02; cleaning in general B08B) · CPC title

• C02F3/348

  characterised by the way or the form in which the microorganisms are added or dosed · CPC title