Antiviral compositions and methods

Patent metadata

Field	Value
Publication number	US-11413320-B2
Application number	US-201916718994-A
Country	US
Kind code	B2
Filing date	Dec 18, 2019
Priority date	Dec 20, 2018
Publication date	Aug 16, 2022
Grant date	Aug 16, 2022

Abstract

The present application relates to a compositions and methods comprising or expressing a MOMO30 protein derived from Momordica balsamina. The MOMO30 protein is about 30 kDa in size, is stable after being autoclaved at 120° C. for 30 min, resists proteolytic cleavage by trypsin, exhibits mannose-sensitive binding HIV gp120, exhibits hemagglutinin and chitinase activity, is capable of activating and stimulating T cell proliferation, is capable of preventing infection by HIV-1 or alleviating symptoms in an HIV-1 infected patients and comprises the amino acid sequence of SEQ ID NO: 1. The MOMO30 protein and/or a nucleic acid encoding the same may be used in methods for preventing or treating viral infections by HIV and other enveloped viruses.

First claim

What is claimed is:

1. A method for preventing or reducing symptoms of HIV infection, comprising orally administering to a subject in need thereof an effective amount of a MOMO30 protein composition prepared by a method comprising the steps of:
(a) drying a plant comprising MOMO30 protein;
(b) lysing cells from the plant in an aqueous medium to form a plant cell lysate;
(c) centrifuging the plant cell lysate to remove debris and particulates to form a clarified plant cell lysate; and
(d) preparing a MOMO30 protein composition from the clarified plant cell lysate so that the MOMO30 protein composition is substantially free of plant components less than 10 kDa in size, and
(e1) passing the clarified plant cell lysate through a molecular weight cut-off filter and collecting the MOMO30-containing retentate; and
(e2) purifying the MOMO30 protein from the clarified plant cell lysate by immunoaffinity purification using an anti-MOMO30 antibody,
wherein the MOMO30 protein composition comprises a MOMO30 protein that is about 30 kDa in size, stable after boiling at 100° C. for 20 min, binds HIV gp120, and comprises the amino acid sequence of SEQ ID NO: 1, and
wherein the MOMO30 protein composition is orally administered to the subject in liquid or dried form.

2. The method of claim 1 , wherein the MOMO30 retentate is eluted in an aqueous buffer to form an aqueous MOMO30 protein composition in solution.

3. The method of claim 2 , wherein the MOMO30 protein composition is dried and administered in a dried form.

4. The method of claim 3 , wherein the dried form is a capsule or tablet.

5. The method of claim 3 , wherein the dried form is administered as an herbal tea to the subject.

6. The method of claim 2 , wherein the MOMO30 solution is added to one or more pharmaceutically acceptable carriers and orally administered to the subject as a liquid.

Assignees

• Morehouse School Of Medicine

Inventors

• Powell Michael D
• Gbodossou Erick Vidjin′ Agnih

Classifications

• A61K9/0053

  Mouth and digestive tract, i.e. intraoral and peroral administration · CPC title

• A61P31/18

  for HIV · CPC title

• A61K9/20

  Pills, tablets, {discs, rods (A61K9/0004, A61K9/0007, A61K9/0056, A61K9/0065 take precedence; for reconstitution of a drink A61K9/0095)} · CPC title

• A61K2236/00

  Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine · CPC title

• A61K38/10 Primary

  Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title