Amino acid-specific binder and selectively identifying an amino acid

Patent metadata

Field	Value
Publication number	US-11390653-B2
Application number	US-202016986334-A
Country	US
Kind code	B2
Filing date	Aug 6, 2020
Priority date	Nov 8, 2018
Publication date	Jul 19, 2022
Grant date	Jul 19, 2022

Abstract

An amino acid-specific binder selectively binds to a binding amino acid. A binder complex selectively identifies the binding amino acid and includes an adjunct attached to the amino acid-specific binder. The adjunct includes a taggant, protein, substrate, or chemical modifier. Selectively identifying an N-terminal amino acid includes anchoring a C-terminal end; contacting an N-terminal amino acid of the anchored analyte with the binder complex; selectively binding when the N-terminal amino acid includes the binding amino acid; producing, by the taggant of the tagged complex, a taggant signal; detecting the taggant signal; and identifying the N-terminal amino acid based on the taggant signal.

First claim

What is claimed is:

1. A binder complex for selectively identifying an amino acid, the binder complex comprising:
an amino acid-specific binder; and
an adjunct attached to the amino acid-specific binder,
wherein the amino acid-specific binder binds selectively to a binding amino acid, and
the amino acid-specific binder comprises the amino acid sequence comprising:
a first amino acid sequence comprising X1-C-P-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
a second amino acid sequence comprising X1-C-S-W-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
a third amino acid sequence comprising X1-P-M-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
a fourth amino acid sequence comprising X1-S-G-R-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
a fifth amino acid sequence comprising X1-P-M-P-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
a sixth amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-S-E-X6-E-X7-G-K-X8;
a seventh amino acid sequence comprising X1-P-R-E-X2-E-X3-N-X4-Q-X5-C-T-X6-Q-X7-A-R-X8;
or an eighth amino acid sequence comprising X1-P-M-S-X2-E-X3-N-X4-Q-X5-S-T-X6-Q-X7-A-R-X8;
wherein:
X1 comprises an amino acid sequence comprising
SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYT (Sequence ID No. 20);
X2 comprises an amino acid sequence comprising FVT;
X3 comprises an amino acid sequence comprising VLKAVF (Sequence ID No. 21);
X4 comprises an amino acid sequence comprising MSED (Sequence ID No. 22);
X5 comprises an amino acid sequence comprising GRRVMMTAHRFGSAVVVV (Sequence ID No. 23);
X6 comprises an amino acid sequence comprising RDIAETKAK (Sequence ID No. 24);
X7 comprises an amino acid sequence comprising ATDL (Sequence ID No. 25); and
X8 comprises an amino acid sequence comprising EAGFPLMFTTEPEE (Sequence ID No. 26),
such that a total percentage amount of substitutions and deletions to X1, X2, X3, X4, X5, X6, X7, and X8 is from 0% to less than 30%, exclusive of
SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMEFVTVVLKAVFRMSEDTGRRVM MTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEPEE (Sequence ID No. 27), or
wherein the amino acid-specific binder comprises the amino acid sequence comprising NLEKIKKLRNVIKEIKKDNIKEADEHEKKE REKETSAWKVILYNDDIHKFSYVTDVIVKVV GQISKAKAHTITVEAHSTGQALILSTQKSKAEKYCQELQQNGLTVSIIHESQLKDKQKK (Sequence ID No. 10).

2. The binder complex of claim 1 , wherein the amino acid sequence comprises:

(Sequence ID No. 1)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCPSFVTVVLK

AVFRMSBDTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDL

GKEAGFPLMFTTEPEE;

(Sequence ID No. 2)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCSWFVTVVLK

AVFRMSEDTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDL

GKEAGFPLMFTTEPEE;

(Sequence ID No. 3)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTVVLK

AVFRMSEDTGRRVMMTAHRFGSAWWCERDIAETKAKEATDLGK

EAGFPLMFTTEPEE;

(Sequence ID No. 4)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTSGRFVTVVLK

AVFRMSEDTGRRVMMTAHRFGSAVWVCERDIAETKAKEATDLG

KEAGFPLMFTTEPEE;

(Sequence ID No. 5)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMPFVTVVLK

AVFRMSEDTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDL

GKEAGFPLMFTTEPEE;

(Sequence ID No. 6)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTVVLK

AVFRMSEDTGRRVMMTAHRFGSAVVWSERDIAETKAKEATDLG

KEAGFPLMFTTEPEE;

(Sequence ID No. 7)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTEVLK

AVFNMSEDQGRRVMMTAHRFGSAVVGVCTRDIAETKAKQATDL

AREAGFPLMFTTEPEE;

(Sequence ID No. 8)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTEVLK

AVFNMSEDQGRRVMMTAHRFGSAVVGVSTRDIAETKAKQATDL

AREAGFPLMFTTEPEE;

or

(Sequence ID No. 10)

NLEKIKKLRNVIKEIKKDNIKEADEHEKKEREKETSAWKVILY

NDDIHKFSYVTDVIVKVVGQISKAKAHTITVEAHSTGQALILS

TWKSKAEKYCQELQQNGLTVSIIHESQLKDKQKK.

Assignees

• Government Of The Us Secretary Of Commerce

Inventors

• Tullman Jennifer A
• Kelman Zvi
• Marino John P

Classifications

• G01N33/582

  with fluorescent label · CPC title

• C07K1/13

  Labelling of peptides · CPC title

• G01N33/6818

  Sequencing of polypeptides · CPC title

• C07K14/195 Primary

  from bacteria · CPC title

• C07K14/445

  Plasmodium · CPC title