Biocompatible oxygen gas generating devices for tissue engineering

Patent metadata

Field	Value
Publication number	US-11389583-B2
Application number	US-202016869751-A
Country	US
Kind code	B2
Filing date	May 8, 2020
Priority date	May 8, 2019
Publication date	Jul 19, 2022
Grant date	Jul 19, 2022

Abstract

The present invention relates to novel biocompatible oxygen gas generating devices that can be implanted into a living subject. In certain embodiments, the oxygen gas generating devices can be used to deliver oxygen gas to tissue in a subject, thereby stimulating tissue growth and repair. In other embodiments, the devices operate by electrolytically splitting endogenous water in a subject. In yet other embodiments, the device further comprises an implantable supercapacitor capable of supplying energy to the oxygen gas generating device.

First claim

What is claimed is:

1. An implantable oxygen generating device comprising:
a. an oxygen generating, biocompatible electrochemical cell,
wherein the electrochemical cell comprises a biocompatible cathode, a biocompatible anode, and a biocompatible hydrogel electrolyte, and
wherein the hydrogel electrolyte is in electrochemical contact with the anode and the cathode of the cell;

b. a biocompatible power source,
wherein the power source is a supercapacitor comprising a plurality of biocompatible electrodes and a biocompatible hydrogel electrolyte, and
wherein the hydrogel electrolyte is in electrochemical contact with each of the plurality of the biocompatible electrodes of the power source;
wherein the biocompatible hydrogel electrolyte in the cell and the biocompatible hydrogel electrolyte in the power source are independently an electrically conductive hydrogel comprising a biopolymer backbone to which an ionic liquid side chain is conjugated; and
wherein the electrochemical cell is in electronic communication with the power source.

2. The device of claim 1 , wherein the biopolymer backbone comprises a biopolymer selected from the group consisting of gelatin, gelatin methacrylate, elastin, hyaluronic acid (HA), alginate, polyethylene glycol (PEG), 2-hydroxyethyl methacrylate (HeMA), poly(ethylene glycol) diacrylate (PEGDA), and poly glycerol sebacate (PGS).

3. The device of claim 1 , wherein the hydrogel electrolyte comprises about 10% to about 20% (w/v) of the biopolymer backbone.

4. The device of claim 1 , wherein the ionic liquid side chain comprises choline.

5. The device of claim 1 , wherein at least one ionic liquid side chain is selected from the group consisting of choline acrylate, choline acetate, choline itaconate, choline salicylate, and any mixtures thereof.

6. The device of claim 1 , wherein the hydrogel electrolyte comprises about 0.1% to about 20% (w/v) of at least one ionic liquid side chain.

7. The device of claim 1 , wherein the hydrogel electrolyte further comprises a photoinitiator selected from the group consisting of lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP), eosin Y, 2-Hydroxy-2-methylpropiophenone, 2-Methyl-4′-(methylthio)-2-morpholinopropiophenone, and 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone.

8. The device of claim 7 , wherein the hydrogel electrolyte comprises about 0.1% to about 1% (w/v) of at least one photoinitiator.

9. The device of claim 1 , wherein the hydrogel electrolyte in the electrochemical cell further comprises a biological cell.

10. The device of claim 9 , wherein the biological cell is selected from the group consisting of mesenchymal stem cell (MSC), cardiomyocyte, and cardiac fibroblast.

11. The device of claim 1 , wherein the electrochemical cell comprises reticular networks of the hydrogel electrolyte.

12. The device of claim 11 , wherein the reticular networks form a grid-like pattern.

13. The device of claim 1 , wherein the biocompatible anode and the biocompatible cathode each independently comprise at least one material selected from the group consisting of graphene hydrogel, cobalt, zinc, silicon nanoparticles, graphene oxide, magnesium, iron, cobalt phosphate, nickel, nickel phosphate, a combination of nickel and phosphate, and a combination of nickel phosphate and cobalt phosphate.

14. The device of claim 1 , wherein the biocompatible anode and biocompatible cathode each comprise cobalt phosphate, graphene hydrogel, and optionally silica nanoparticle/laponite.

15. The device of claim 1 , wherein the plurality of electrodes comprises graphene hydrogel.

16. The device of claim 15 , wherein the plurality of electrodes further comprises silica nanoparticles/laponite.

17. The device of claim 1 , wherein the biocompatible anode and biocompatible cathode each independently comprise about 1% to about 5% (w/v) of cobalt phosphate.

18. The device of claim 1 , wherein the device has at least one of the following characteristics: is biodegradable, is biocompatible, is operational at about neutral pH.

19. The device of claim 1 , wherein the electrochemical cell and the power source are 3D-printed.

20. The device of claim 1 , wherein the power source comprises an interdigitated structure comprising the hydrogel electrolyte and the plurality of biocompatible electrodes.

21. A method of treating a tissue injury in a subject, the method comprising:
subcutaneously implanting the device of claim 1 into the subject, wherein the device is implanted on, near, or in close proximity to the tissue injury.

22. The method of claim 21 , wherein the tissue injury is at least one selected from the group consisting of an ischemic reperfusion injury, muscle injury, pancreatic tissue injury, and neural tissue injury.

23. An oxygen generating biocompatible device comprising
a. a biocompatible electrochemical cell comprising a graphene hydrogel/cobalt-phosphorous (GH/Co—P) alloy cathode, a graphene hydrogel/cobalt phosphate (GH/CoP i ) anode, and a biocompatible, conductive hydrogel electrolyte, wherein the anode and the cathode further comprise silica nanoparticles/laponite, and
wherein the hydrogel electrolyte is in electrochemical contact with the biocompatible anode and the biocompatible cathode;
b. a power source comprising a plurality of electrodes comprising a graphene hydrogel and laponite, the biocompatible conductive hydrogel electrolyte wherein each of the plurality of electrodes is in electrochemical contact with a biocompatible conductive hydrogel electrolyte,
wherein the biocompatible electrochemical cell and the power source are in electronic communication with each other; and
wherein each biocompatible conductive hydrogel electrolyte is independently an electrically conductive hydrogel comprising a biopolymer backbone to which an ionic liquid side chain is conjugated.

24. The device of claim 23 , wherein the biopolymer backbone comprises a biopolymer selected from the group consisting of gelatin, gelatin methacrylate, elastin, hyaluronic acid (HA), alginate, polyethylene glycol (PEG), 2-hydroxyethyl methacrylate (HeMA), poly(ethylene glycol) diacrylate (PEGDA), and poly glycerol sebacate (PGS).

25. The device of claim 23 , wherein the ionic liquid side chain comprises choline.

26. The device of claim 23 , wherein the ionic liquid side chain is selected from the group consisting of choline acrylate, choline acetate, choline itaconate, choline salicylate, and mixtures thereof.

Assignees

• Univ Rowan

Inventors

• Noshadi Iman

Classifications

• Y02E60/10

  Energy storage using batteries · CPC title

• H01M4/133

  Electrodes based on carbonaceous material, e.g. graphite-intercalation compounds or CFx · CPC title

• A61L31/06

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds {(A61L31/041 takes precedence)} · CPC title

• C25B1/02

  Hydrogen or oxygen · CPC title

• B29C64/106

  using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material · CPC title