Serine proteases of the Bacillus gibsonii-clade

Patent metadata

Field	Value
Publication number	US-11384348-B2
Application number	US-201916703053-A
Country	US
Kind code	B2
Filing date	Dec 4, 2019
Priority date	Dec 13, 2013
Publication date	Jul 12, 2022
Grant date	Jul 12, 2022

Abstract

The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.

First claim

We claim:

1. A recombinant polypeptide or an active fragment thereof of the Bacillus Gibsonii -clade, wherein the recombinant polypeptide or active fragment thereof has proteolytic activity and comprises an amino acid sequence having at least 97% identity to the amino acid sequence of SEQ ID NO: 69, wherein said Bacillus Gibsonii -clade comprises a DXGIXXHSDLXXXGGASXXXXXPTTADLNXHGTH (SEQ ID NO:47) or DXGIXXHSDLXXXGGASXXXXXXTTADLXXHGTH (SEQ ID NO:90) motif, wherein the initial D is the active site Aspartic acid residue and the penultimate H is the active site Histidine, and X is any amino acid.

2. The recombinant polypeptide or active fragment thereof of claim 1 , wherein the Bacillus Gibsonii -clade comprises SEQ ID NO:47.

3. The recombinant polypeptide or active fragment thereof of claim 1 , wherein the Bacillus Gibsonii -clade comprises SEQ ID NO:90.

4. The recombinant polypeptide or active fragment thereof of claim 1 , wherein the polypeptide has protease activity in the presence of a surfactant.

5. The recombinant polypeptide or active fragment thereof of any preceding claim, wherein the polypeptide retains at least 50% of its maximal protease activity at a pH range of 8 to 12 or a temperature range of 50° C. to 75° C.

6. The recombinant polypeptide or active fragment thereof of claim 1 , wherein the polypeptide has cleaning activity in a detergent composition.

7. The recombinant polypeptide of claim 6 , wherein the detergent composition is an automatic dish washing detergent or a laundry detergent.

8. The recombinant polypeptide of claim 7 , wherein the cleaning activity comprises hydrolysis of an egg yolk substrate or a substrate selected from the group consisting of blood, milk, ink and combinations thereof.

9. A composition comprising a surfactant and the recombinant polypeptide of claim 1 .

10. The composition of claim 9 , wherein the composition is a detergent composition.

11. The composition of claim 10 , wherein said composition further comprises at least one calcium ion and/or zinc ion, at least one stabilizer, from about 0.001% to about 1.0 weight % of said recombinant polypeptide, at least one bleaching agent, at least one adjunct ingredient, and/or one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, metalloproteases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, perhydrolase, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, xylosidases, metalloproteases, additional serine proteases, and combinations thereof.

12. The composition of claim 9 , wherein said composition is phosphate-free or contains phosphate and/or is borate-free or contains borate.

13. A method of cleaning comprising contacting a surface or an item with the composition of claim 9 .

14. A polynucleotide comprising a nucleic acid sequence:
(i) encoding an amino acid sequence of SEQ ID NO:47 or 49;
(ii) encoding an amino acid sequence of SEQ ID NO:47 or 49 and further encoding an amino acid sequence having 97% identity to an amino acid sequence of SEQ ID NO:
(iii) encoding an amino acid sequence having 70% identity to an amino acid sequence of SEQ ID NO:
(iv) having at least 90% identity to SEQ ID NO: or
(xi) having complementarity to SEQ ID NO:.

15. An expression vector comprising the polynucleotide of claim 14 .

16. A host cell transformed with the vector of claim 15 .

17. A composition comprising the polypeptide of claim 1 , wherein said composition is selected from a textile processing composition, leather processing composition, feather processing composition, wound cleaning composition, and a contact lens cleaning composition.

Assignees

• Danisco Us Inc

Inventors

• Kolkman Marc
• Goedegebuur Frits
• Babe Lilia Maria
• Mejldal Rie
• Mulder Harm
• Kellett-Smith Anja Hemmingsen
• Bott Richard R
• Scotcher Miles Christopher

Classifications

• C11D3/386

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• C12N9/54 Primary

  bacteria being Bacillus · CPC title

• A47L15/0002

  Washing processes, i.e. machine working principles characterised by phases or operational steps · CPC title

• C12Y304/21

  Serine endopeptidases (3.4.21) · CPC title

• A47L2601/20

  Other treatments, e.g. dry cleaning · CPC title