Method for producing intestinal organoid derived from pluripotent stem cells
US-2020157507-A1 · May 21, 2020 · US
US11371013B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11371013-B2 |
| Application number | US-202016801785-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 26, 2020 |
| Priority date | Jul 24, 2012 |
| Publication date | Jun 28, 2022 |
| Grant date | Jun 28, 2022 |
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The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.
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The invention claimed is: 1. A method of producing a sphere, comprising cultivating an adherent cell in a suspended state in a liquid medium composition comprising deacylated gellan gum or a salt thereof.
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