Anti-TIGIT antibody and use thereof

Patent metadata

Field	Value
Publication number	US-11370837-B2
Application number	US-201916965687-A
Country	US
Kind code	B2
Filing date	Jul 25, 2019
Priority date	Jul 25, 2018
Publication date	Jun 28, 2022
Grant date	Jun 28, 2022

Abstract

The present invention relates to a novel antibody and an antibody fragment thereof that specifically bind to TIGIT and a composition comprising the antibody or the antibody fragment. In addition, the present invention relates to a nucleic acid encoding the antibody or the antibody fragment thereof, a host cell comprising the nucleic acid, and related use. Furthermore, the present invention relates to therapeutic and diagnostic use of the antibody and the antibody fragment.

First claim

The invention claimed is:

1. An antibody or an antigen-binding fragment thereof that binds to TIGIT, comprising three heavy chain complementarity determining regions (HCDRs) and three light chain complementarity determining regions (LCDRs), wherein
(a) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 1, 2, 3, 4, or 5, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 6, 7, 8, 9, or 10, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 11, 12, 13, 14, or 15, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 16, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 17, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 18;
(b) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 19, 20, or 21, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 22, 23, or 24, HCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 25, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 26, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 27, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 28, 29, or 30;
(c) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 31, 32, or 33, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 34, 35, 36, or 37, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 38, 39, or 40, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 41, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 42, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 43;
(d) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 44, 45, 46, or 47, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 48, 49, 50, 51, or 52, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 53, 54, 55, or 56, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 57, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 58, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 59;
(e) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 60, 61, or 62, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 6, 63, 64, or 65, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 66, 67, or 68, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 69, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 17, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 70;
(f) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 71, 72, 73, or 74, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 22, 75, 76, or 77, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 78, 79, or 80, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 81, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 82, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 83;
(g) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 178, 179, 180, or 181, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 6, 7, 8, or 9, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 182, 183, 184, or 185, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 16, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 17, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 18;
(h) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 186 or 187, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 22 or 23, HCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 188, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 26, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 27, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 28 or 29;
(i) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 189 or 190, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 34, 35 or 36, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 191 or 192, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 41, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 42, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 43;
(j) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 193, 194, or 195, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 48, 49, 50, or 51, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 196, 197, or 198, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 57, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 58, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 59; (k) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 199 or 200, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 6, 63 or 64, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 201 or 202, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 69, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 17, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 70; or
(1) HCDR1 comprises an amino acid sequence set forth in SEQ ID NOs: 203, 204, or 205, HCDR2 comprises an amino acid sequence set forth in SEQ ID NOs: 22, 75, or 76, HCDR3 comprises an amino acid sequence set forth in SEQ ID NOs: 206 or 207, LCDR1 comprises an amino acid sequence set forth in SEQ ID NO: 81, LCDR2 comprises an amino acid sequence set forth in SEQ ID NO: 82, and LCDR3 comprises an amino acid sequence set forth in SEQ ID NO: 83.

2. The antibody or the antigen-binding fragment thereof according to claim 1 , comprising
(i) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 84, 85, 86, or 87, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 104,
(ii) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 88, 89, or 90, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 105 or 106,
(iii) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 91, 92, or 93, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 107,
(iv) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 94, 95, 96, or 97, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 108,
(v) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 98, 99, or 100, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 109, or
(vi) a heavy chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 101, 102, or 103, and/or a light chain variable region comprising an amino acid sequence having at least 80%, 85%, or 90% sequence identity to an amino acid sequence set forth in SEQ ID NO

Assignees

• Innovent Biologics Suzhou Co Ltd

Inventors

• Shi Xinzhen
• Zhang Pan
• Liu Junjian

Classifications

• A61K39/00

  Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) · CPC title

• C07K14/70503

  Immunoglobulin superfamily · CPC title

• C07K14/705

  Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title

• A61P31/12

  Antivirals · CPC title

• G01N33/53

  Immunoassay; Biospecific binding assay; Materials therefor · CPC title