Systems and methods for manufacturing a microfluidic rotor device

Patent metadata

Field	Value
Publication number	US-11370177-B2
Application number	US-201916547697-A
Country	US
Kind code	B2
Filing date	Aug 22, 2019
Priority date	Aug 24, 2018
Publication date	Jun 28, 2022
Grant date	Jun 28, 2022

Abstract

Described herein are various embodiments directed to rotor devices, methods, and systems. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. A method may include bonding a first layer and a second layer using two-shot injection molding. The first layer coupled to the second layer may collectively define a set of wells. The first layer may be substantially transparent. The second layer may define a channel. The second layer may be substantially absorbent to infrared radiation. A third layer may be bonded to the second layer using infrared radiation. The third layer may define an opening configured to receive a fluid. The third layer may be substantially transparent. The channel may establish a fluid communication path between the opening and the set of wells.

First claim

The invention claimed is:

1. A method, comprising:
manufacturing a microfluidic rotor device by bonding a first layer and a second layer using two-shot injection molding, wherein the first layer coupled to the second layer collectively defines a set of wells, the first layer being substantially transparent, and the second layer defining a channel, the second layer being substantially absorbent to infrared radiation;
disposing a lyophilized reagent in one or more wells of the set of wells;
aligning the second layer to a third layer after the bonding of the first layer and the second layer;
aligning a photomask to the third layer after the bonding of the first layer and the second layer, the photomask configured to block the infrared radiation to one or more portions of the second layer; and
bonding the third layer to the second layer using infrared radiation via an infrared laser beam, the photomask configured to block the infrared laser beam over the lyophilized reagent, and the third layer defining an opening configured to receive a fluid, the third layer being substantially transparent, wherein the channel establishes a fluid communication path between the opening and the set of wells.

2. The method of claim 1 , further comprising bonding a fourth layer to the third layer, the fourth layer being at least partially opaque.

3. The method of claim 2 , wherein bonding the fourth layer to the third layer comprises ultrasonically welding the fourth layer to the third layer.

4. The method of claim 2 , wherein bonding the fourth layer to the third layer may include one or more of laser welding, adhesive bonding, and solvent bonding.

5. The method of claim 1 , wherein the second layer includes at least about 0.1% by weight of carbon black.

6. The method of claim 5 , wherein the second layer includes a laser absorbing dye substantially absorbent to radiation between about 750 nm to about 3,000 nm.

7. The method of claim 1 , wherein the infrared radiation includes a wavelength of about 940 nm.

8. The method of claim 1 , wherein the first layer and the third layer are each independently transparent.

9. The method of claim 1 , wherein substantially transparent includes light transmission of at least one of ultraviolet light, visible light, and infrared radiation.

10. The method of claim 1 , wherein the second layer is substantially absorbent to at least one of mid-infrared radiation and near-infrared radiation.

11. The method of claim 1 , wherein substantially absorbent to infrared radiation includes absorbing infrared radiation in a sufficient amount within a predetermined period of time to transition the second layer from a solid phase to a molten phase.

12. The method of claim 1 , wherein the second layer is substantially absorbent to at least 940 nm wavelength radiation.

13. The method of claim 1 , wherein the first layer, the second layer, and the third layer are independently composed of one or more of acrylic, polycarbonate, cyclic olefin copolymers (COC), and acrylonitrile butadiene styrene (ABS).

14. The method of claim 1 , wherein the second layer includes between about 0.2% to about 0.4% by weight of carbon black.

15. The method of claim 1 , further comprising bonding the third layer to the second layer including using a line beam of infrared radiation.

16. The method of claim 1 , further comprising forming a set of shut offs by engaging a first half of a mold with the first layer disposed in a second half of the mold.

17. The method of claim 1 , further comprising forming the third layer using injection molding.

Assignees

• Zoetis Services Llc

Inventors

• Shartle Robert Justice
• Trigub Gregory

Classifications

• B29C45/16

  Making multilayered or multicoloured articles {(B29C45/0062 takes precedence; feeding colouring materials into the injection unit B29C45/1816)} · CPC title

• B29C65/1677

  making use of an absorber or impact modifier · CPC title

• G01N21/07

  Centrifugal type cuvettes (G01N21/09 takes precedence) · CPC title

• B29C65/50

  using adhesive tape {, e.g. thermoplastic tape; using threads or the like (B29C65/3444 takes precedence)} · CPC title

• B29C65/1696

  making use of masks · CPC title